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Chlorpyrifos ethyl' in rat pankreası üzerine etkisi

The effects of chlorpyrıfos-ethyl on pancreas in rats

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2005

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Pancrease is an organ whic h realtively vulnareble to various xenobiotics. It is reported that organophospahates cause acute pancreatitis at approximately 12% rate. We aimed to investigate effects of chlorpyrifos-ethyl (CE) [O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)phosphorothioate], an organophosphate insecticide, on pancrease at the maximum tolerable dose within various duration. Study groups designed as control group, CE treated (administered single tolerable dose) adult group and CE treated (administered single tolerable dose) infantil group. Each group divided into three subgroups. Animals were sacrificied at days 2., 7. and 14. We examined effects of CE on serum activities of cholinesterase (ChE), amylase and lipase enzymes in plasma and, blood levels of malondialdehyde (MDA) in erythrocytes. Compared to control group, ChE activity was found to be significantly decreased while amylase activity and MDA level were found to be sigificantly elevated in CE treated group. Compared to CE treated group, lipase activity was found to be increased in CE treated group. Based on the findings that were obtained from this study we can conclude that maximum tolerable dose of CE may cause pancreas disorder that is probably not directly related to increased oxidative stress or inhibition of cholinesterase enzyme activity.
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Abstract (Original Language): 
Pankreas bazı ksenobiotiklere göreceli olarak daha hassas bir organdır. Organofosfat zehirlenmelerinde ortalama % 12 oranında akut pankreatite rastlandığı bildirilmiştir. Bu çalışmada organofosfat insektisitlerden chlorpyrifos ethyl (CE)'in tolere edilebilir maksimum dozda pankreas üzerine olası etkilerini genç ve erişkin ratlarda araştırmayı planladık. Çalışma grupları; genç kontrol grubu, genç CE verilen grup ve erişkin CE verilen grup şeklinde düzenlendi. Bu gruplarda üçer alt gruba ayrılarak deney başladıktan sonra 2, 7 ve 14 gün sonra kan örnekleri alındı. Her grubun deney süresinin bittiği günde kan örnekleri alınarak plazmada kolinesteraz (ChE), amilaz ve lipaz aktiviteleri ile malondialdehit (MDA) düzeyleri ölçüldü. Kontrol grubuyla karşılaştırıldığında CE uygulanan grubun ChE aktivitesinin anlamlı olarak azaldığı görüldü. CE uygulanan grup ile kontrol grubu karşılaştırıldığında amilaz aktivitesinin ve MDA düzeyinin anlamlı olarak arttığı görüldü. Sonuç olarak bu bulgulardan CE'nin maksimum tolare edilebilir dozda ratlara uygulandığında hafif pankreas hasarına neden olabileceği fakat bunun oksidatif stres artışı veya kolinesteraz enzim aktivitesin inhibisyonu ile doğrudan yakın ilişki halinde olmadığı düşünüldü.
FULL TEXT (PDF): 
PDF icon arastirmax-chlorpyrifos-ethyl-rat-pankreasi-uzerine-etkisi.pdf
  • 4
19-22
  • Turkish

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