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Klorpirifosun sıçanlarda öğrenmeye etkileri

The effects of chlorpyrıfos on learnıng ın rats

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Aim: In this study we try to determine whether chlorpyrifos (CPp) administration affetcs the learning and relation of learning with acetylcholine esterase (AChE) levels. Materials and methods: We arrange 4 main groups as adult CPp, juvenile CPp, adult control and juvenile control group. Rats were trained on the working memory task in an eight arm radial maze. After acquisition they underwent enjections of CPp and control group. Juvenile CPp group received 40 mg/kg CPp and adult CPp group received 70 mg/kg CPp subcutaneously. Each group were seperated into 3 groups again as 2nd, 7th and 14th groups. Learning tests were applied by using radial arm maze on 2nd, 7th and 14th days. Choice Accuracy and Response Latency parameters were calculated according to the data provided from these tests. Also AchE activities of rats were studied on these days. pindings: CPp caused a significant increase in all of the adult rats where as on 2nd and 7th days in juvenile ones. It did not cause any significant difference on Choice Accuracy and Response Latency. Results: It is concluded that the AChE inhibition of chlorpyrifos given at single dose did not affect the learning in juvenile and adult rats, therefore there is no direct relation between CPp and learning; the outcome is that future studies with different doses and administration durations may provide more explanatory results.
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Abstract (Original Language): 
Amaç: Klorpirifosun (CPp) öğrenmeyi etkileyip etkilemediği ve öğrenmenin asetilkolin esteraz (AChE) düzeyleri ile ilişkisi çalışmamızda ortaya konulmaya çalışılmıştır. Gereç ve yöntem: Genç ve erişkin CPp grupları ve genç ve erişkin kontrol grupları olmak üzere 4 ana grup oluşturuldu. Tüm gruplar ışınsal kollu labirentte eğitildiler. Daha sonra CPp enjeksiyonu ve kontrol grubu enjeksiyonuna başlandı. Genç CPp grubuna 40 mg/kg cilt altı, erişkin CPp grubuna 70 mg/kg cilt altı CPp enjeksiyonu yapıldı. Her grup kendi içinde tekrar 2.gün, 7.gün ve 14.gün grubu olmak üzere ayrıldı. 2., 7. ve 14. günlerde ışınsal kollu labirentte öğrenme testleri yapılmıştır. Bu test bilgileri kullanılarak Seçim Doğruluğu ve Cevap Gecikmesi parametreleri hesap edilmiştir. Bununla beraber bu günlerde sıçanların AChE aktiviteleri çalışılmıştır. Bulgular: CPp, AChE düzeylerini erişkin sıçanlarda tüm günlerde, genç sıçanlarda ise 2. ve 7. günlerde anlamlı olarak düşürmüştür. Işınsal kollu labirentte Seçim Doğruluğu ve Cevap Gecikmesi üzerinde ise anlamlı bir değişikliğe neden olmamıştır. Sonuç: Uyguladığımız tek doz CPp ile AChE enzim inhibisyonunun genç ve erişkin sıçanlarda öğrenmeyi etkilemediği, dolayısıyla öğrenme ile direkt ilişkisinin olmayabileceği düşünülmüş; farklı doz ve uygulama süresi ile yeni çalışmaların yapılmasıyla daha açıklayıcı sonuçların elde edilebileceği kanaatine varılmıştır.
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REFERENCES

References: 

1. Agrawal D, Sultana P, Gupta GS. Oxidative damage and changes in the glutathione redox system in erythrocytes from rats treated with hexachlorocyclohexane. Food Chem Toxicol. 1991;29(7):459-62.
2. Levin ED, Andy N, Baruah A, Elias A, Christopher NC, Seidler FJ, Slotkin TA. Prenatal chlorpyrifos exposure in rats causes persistent behavioral alterations. Neurotoxicol Teratol. 2002; 24 (6): 733-741.
3. Icenogle LM, Christopher NC, Blackwelder WP, Caldwell DP, Qiao D, Seidler FJ, Slotkin TA, Levin ED. Behavioral alterations in adolescent and adult rats caused by a brief subtoxic exposure to chlorpyrifos during neurulation. Neurotoxicol Teratol. 2004; 26 (1):95-101.
4. Richardson RJ. Assessment ofthe neurotoxic potential ofchlorpyrifos relative to other organophosphorous compounds: a critical review ofthe literature. J Toxicol Environ. Health. 1995; 44(2): 135-165.
5. Prior H, Schwegler H, Ducker G. Dissociation of spatial reference memory, spatial working memory, and hippocampal mossy fiber distribution in two rat strains differing in emotionality. Behav Brain Res. 1997; 87: 183-194, 1997.
6. White AM, Best PJ. The effects of MK-801 on spatial working memory and within-session spatial learning. Pharmacol Biochem Behav. 1998; 59: 613-617.
7. Levin ED, Andy N, Nakajima A, Christopher NC, Seidler FJ, Slotkin TA. Persistent behavioral consequences ofneonatal chlorpyrifos exposure in rats. Brain Res Dev Brain Res. 2001; 130 (1): 83-89.
8. Pope CN, Chakraborti TK, Chapman ML, Farrar JD.
S.D.Ü. Tıp pak.
Derg
. 2008:15(3)/1-6
6
Şavik, Klorpirifos ve öğrenme
Long-term neurochemical and behavioral effects induced by acute chlorpyrifos treatment. Pharmacol BiochemBehav. 1992 ;42(2):251-6.
9. Liu J, Pope CN. Comparative presynaptic neurochemical changes in rat striatum following exposure to chlorpyrifos or parathion. J Toxicol Environ HealthA. 1998 10;53(7):531-44.
10. Rawlins NP, Deacon MJ. Further developments of maze procedures. Saghal A. In: Behavioral Neuroscience A Practical Approach Volume I. Oxford UniversityPress, 1993; 95-106.
11.
Gülteki
n F, Delibaş N, Kutluhan S, Akdoğan M, Kılınç İ, SütçüR. Ratbeyindokulanndachlorpyriphos-ethyl'in neden olduğu antioksidan sistemdeki değişiklikler ile melatonin ve vitamin C + vitamin E'nin koruyucu etkileri. S.Ü. Tıp Fak. Derg., 2001; 17:
79-86.
12. Buznikov GA, Nikitina LA, Bezuglov VV, Lauder JM, Padilla S and Slotkin TA. An intervertebrate model of the developmental neurotoxicity ofinsecticides: Effects ofchlorpyrifos and dieldrin in sea urchin embryos and larvae. Environmental Health Perspectives. 2001; 109(7): 651-661.
13. Slotkin TA, Developmental cholinotoxicants: nicotine andchlorpyrifos, Environ. Health Perspect. 1999; 107 (Suppl. 1) 71-80.
14. Dam K, Garcia SL, Seidler FJ, Slotkin TA, Neonatal chlorpyrifos exposure alters synaptic development and neuronal activity in cholinergic and catecholaminergic pathways, Dev Brain Res. 1999; 116 9-20.
15. Campbell, CG, SeidlerFJ, and SlotkinTA. Chlorpyrifos interferes with cell development in rat brain regions. BrainResBull. 1997;43, 179-189.
16. Garcia SJ, Seidler FJ, Crumpton TL, and Slotkin TA. Does the developmental neurotoxicity ofchlorpyrifos involve glial targets? Macromolecule synthesis, adenylyl cyclase signaling, nuclear transcription factors, and formation ofreactive oxygen in C6 glioma cells. Brain Res. 2001;891: 54-68.
17. Sanchez-Santed F, Canadas F, Flores P, Lopez-Grancha M, Cardona D. Long- term functional neurotoxicity of paraoxon and chlorpyrifos: behavioral and pharmacological evidence. Neurotoxicol Teratol, 2004; 26 : 305-317.
18. Maurissen JP, Shankar MR, Mattsson JL. Chlorpyrifos: lack ofcognitive effects in adult Long-Evans rats. Neurotoxicol Teratol. , 2000; 22 (2):237-246.
19. Maurissen JP, Hoberman AM, Garman RH, Hanley TR Jr. Lack of selective developmental neurotoxicity in rat pups from dams treated by gavage with chlorpyrifos. Toxicol Sci. , 2000; 57 (2): 250-263.
20. Kaplan JG, Kessler J, Rosenberg N, Pack D and Schumburg HH. Sensory neuropathy associated with DURSBAN (chlorpyrifos) exposure. Neurology., 1993;
43:2193-2196.
21. Stanton ME, Mundy WR, Ward T, Dulchinos V and Barry CC. Time dependent effects ofacute chlorpyrifos administration on spatial delayed alteration and cholinergic neurochemistry in weanling rats. Neurotoxicology. 1994; 15(1): 201-208.
22. Bushnell PJ, Kelly KL, Ward TR. Repeated inhibition ofcholinesterase by chlorpyrifos in rats: behavioral, neurochemical and pharmacological incides oftolerance. J Pharmacol Exp Ther. 1994; 270 (1):15-25.
23. Terry AV Jr, Stone JD, Buccafusco JJ, Sickles DW, Sood A, Prendergast MA. Repeated exposures to subthreshold doses ofchlorpyrifos in rats: hippocampal damage, impaired axonal transport, and deficits in spatial learning. J Pharmacol Exp Ther. 2003; 305 (1):
375-384.
24. Canadas F, Cardona D, Davilla E, Sanchez-Santed F. Long-term neurotoxicity ofchlorpyrifos: spatial learning impairment on repeated accusition in a water maze. Toxicol Sci. 2005; 85 (5): 944-951.
25. Levin ED, Persisting effects ofchronic adolescent nicotine administration on radial-arm maze learning and response to nicotinic challenges. Neurobehavioral Teratology Society Annual Meeting Keystone, CO,
1999

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