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DENEYSEL DIYABETIK NEFROPATIDE ENALAPRIL VE L KARNITIN ETKILERI

EFFECTS OF ENALAPRIL AND L-CARNITINE IN EXPERIMANTAL DIABETIC NEPHROPATHY

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Abstract (2. Language): 
Hyperglisemia, increased angiotensin II ATİ reseptör activity, oksidative stress, decreasing intracellular A TP play an important rol in occurance diabetic nephropathy (DN). In our study, effects of angiotensin converting enzyme inhibitor (AÇEi) as enalapril (EN), and L-Carnitine (LC) were investigated on DN in streptozotocin-induced diabetic rats. Except healthy, patient control (HK, PK) groups, 2.5 mg/kg/day EN and 100 mg/kg/day LC were given in a water for six weeks. After 24 hrs urine collection, blood with-drowned by cardiacpunction then, rats were sucrified. Erythrocytes count (Er), hemoglobin (Hb), hematocrit (Hct) in blood; and glucose, HbA 1c, urea, creatinine (pCr), uric acid (UA), P04, Ca2", Na, K, albumin (Alb) inplasma; superokside dismutase enzyme (SOD) as an antioxidant, malonyl dialdehyte (MDA) as lipidperoxidation product (ROS) were measured in eryhtrocyte were measured. Daily excretion of urinary glucose (UG), albumin (UAE), N-acetyl-b-D-glucosaminidase (NAG) were measured, immunuhistochemical kollagene type IV accumulation and renal histopathology were investigated on light microscopy. After STZ injection DN was established with increased glucose, HbAlc, UG, UAE, NAG (P<000), urea, pKr (P<005). According to PKgroup, lower UG (P<.000), NAG, urine volume, PO4, and higher Alb (P<.05) in EN group, higher Er, Hb, lower urine volume, UG, NAG (P<.05) in LC groups were found. According to HK, nonsignificant increased MDA in PK, unchanged in EN and LC groups were found. Tubulo-glomerular basal membrane thickness and accumulation tip IV /collagen as DN findings significant in PK, moderate EN, and mild degree in LC group were found. Our findings suggests that, LC have protective effect on diabetic nephropathy like ACEi.
Abstract (Original Language): 
Diabetik nefropati (DN) oluşmasında hiperglisemi, anjiotensin II ATİ reseptör aktivasyon ve oksidatif stres artışı, hücre içi ATP azalması önemli rol alır. Çalışmamızda, streptozosin (STZ) ile diyabet oluşturulan raflarda anjiotensin /converting enzim inhibitörü (ACEi) olan Enalapril (EN) ve L-Karnitin (LK) in DN ye etkileri araştırıldı. Dokuz ratlık gruplarda, sağlıklı (SK) ve hastalıklı (HK) kontrol dışındakilere içme suyu ile 2.5mg/ kg gün ENve 100 mg/kg/gün LKaltı hafta verildi. Çalışma sonunda 24st lik idrar toplandı, kardiak ponksiyonla kan alınarak ratlar sakrifıye edildi. Kanda; eritrosit (Er) sffyısı, hemoglobin (Hb), hematokrit (Hct), plazmada; glukoz, HbAlÇ üre, kreatinin (pKr), ürik asit (ÜA), P04, Ga , Na^, K\ albumin (Alb) ve Er içi antioksidan enzim olaraksüperoksit dismutase (SOD) ve lipidperoksidasyon ürünü (ROS) malonyl dialdehid (MDA) ölçüldü. İdrarla atılan glukoz (UG), albumin (UAE) ve N-acetyl-B-D glukozaminidase (NAG) incelendi. Böbrek dokusu ışık mikroskobunda ve immunohistokimyasal Tip IVkollajen birikimi incelendi. STZ ile glukoz, HbAlc, UG, UAE, NAG(P<.000), üre, pKr (P<.005) artışı ile DN oluştu. HK grubuna göre, EN grubunda UG (P<.000), NAG, idrar volümü ve P04 düşük (P<.05), Alb yüksek (P<.05) ve LK grubunda Er, Hb yüksek, UG, NAG ve idrar volümü düşük (P<.05) bulundu. SK'e göre MDA değeri HK grubunda anlamsız arttı, EN ve LK grubunda değişmedi. DN bulguları olan glomerüler ve tubuler bazal membranda kalınlaşma ve tip IV kollajen birikimi HK'da belirgin, EN'de orta, LK grubunda hafif derecede bulundu. Bulgularımız, ACEi 'ne benzer şekilde LK'in DN gelişimini azaltıcı etki yaptığını düşündürmektedir.
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