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Hemodiyaliz Hastalarında Farklı Diyaliz Membranlarının Polimorfonükleer Lökosit Oksidatif Stresi Üzerine Etkisi

Effect of Different Dialyzer Membranes on the Oxidative Burst Status of Poylymorphonuclear Leukocytes in Hemodialysis Patients

Journal Name:

  • Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
In this study, we aimed to investigate whether there is an abnormality in intracellular antioxidant enzyme activities of polymorphonuclear leukocytes (PMNL) of end-stage renal disease (ESRD) patients who had been treated with hemodialysis and how do the different types of dialyzer membranes affect these enzyme activities. Fifteen ESRD patients undergoing hemodialysis treatment were enrolled into the study and they were dialyzed with polysulphone (P) and cupropha-ne (C) membranes in two consecutive hemodialysis sessions. Superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in PMNLs from the venous blood samples of the patients, obtained before and after each hemodialysis session, were determined. A control group consisting of 15 healthy subjects was also included. Predialysis PMNL SOD (p-0.285 for P-dialysİs, p=0.512 for C dialysis) and GSH-Px (p=0.285 for P-dialysis, p=0.486 for C-dialysis) activities of the patients were not different from those of the controls. Similiarly, postdialysis PMNL SOD (p=0.838 for P-dialysis, p-0.744 for C-dialysis) and GSH-Px |p=0.512 for P-dialysis, p=0.870 for C-dialysis) activities of the patients did not show difference from control data. Hemodialysis with both types of membranes did not cause a statistically significant change in PMNL SOD (p=0.460 for P-dialysis, p-0.532 for C-dialysis) and GSH-Px (p=0.773 for P-dialysis, p=0.570 for C-dialysis} activities. In conclusion, there is no difference between ESRD patients undergoing hemodialysis and controls with respect to PMNL oxidative stress status.
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Abstract (Original Language): 
Bu çalışmada, son dönem böbrek yetmezlikli (SDBY) hemodiyaliz hastalarının nötrofil (PMNLI intraselüler antioksidan enzim aktivi-teleri ve farklı diyaliz membranlarının bu enzimler üzerindeki etkilerinin incelenmesi amaçlanmıştır. Çalışmaya SDBY mevcut 15 hasta ve kontrol grubu olarak 15 sağlıklı gönüllü dahil edilmiştir. Hastalar po-lisülfon (P) ve kuprofan (C) membranları ile diyalize alınmış, her bir diyaliz öncesi ve sonrası, venöz kanda nötrofil süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) aktiviteleri saptanmıştır. Hastaların prediyaliz PMNL SOD (p=0.285 P-diyaliz, p=0.512 C-diyaliz) ve GSH-Px (p=0.285 P-diyalîz, p=0.486 C-diyaliz) aktiviteleri ile post-diyaliz PMNL SOD {p=0.838 P-diyaliz, p=0.744 C-diyaliz) ve GSH-Px (p=0.512 P-diyaliz, p=0.870 C-diyaliz) aktiviteleri kontrol grubundan farklı bulunmamıştır. Farklı hemodiyaliz membranları kullanımıyla PMNL SOD (p=0.460 P-diyaliz, p-0.532 C-diyaliz) ve GSH-Px (p=0.773 P-diyaliz, p=0.570 C-diyaliz) aktivitelerinde, diyaliz sonrası dönemde diyaliz Öncesi döneme göre istatistiksel olarak belirgin bir farklılık saptanmamıştır. Sonuç olarak, son dönem böbrek yetmezlikli hemodiyaliz hastalarının PMNL oksidatif stres durumları kontrol vakaları-nınkinden farklı değildir. Bunun yanında, sentetik (polisülfon) veya selüloz (kuprofan) membran kullanımı, PMNL SOD ve GSH-Px enzim aktivitelerini etkilememektedir.
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  • 1
26-29
  • Turkish

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