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Güneydoğu Anadolu Bölgesinde Son Dönem Böbrek Yetmezliği Hastalarında ACE Gen Polimorfizmi Prevalansı

Prevalance of ACE Gene Polymorphism in Patients with End Stage Kidney Disease in Southeastern Turkey

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Abstract (2. Language): 
Aim: To evaluate the frequency of angiotensin converting enzyme gene polymorphism from renin angiotensin aldosterone system in patients undergoing chronic dialysis. Materials and Methods: Eighty-seven patients undergoing dialysis treatment and 38 healthy volunteers were enrolled in the study. Demographic features, arterial blood pressure, body mass index (BMI), hematological and biochemical parameters were recorded. ACE gene polymorphism was studied using sterile polymerase chain reaction (PCR) method from blood samples taken into tubes containing EDTA. Results: Of the 87 patients, 49 were undergoing hemodialysis and 38 were on peritoneal dialysis programme. In the patient group male/female ratio was 48/39 and the mean age was 41.1 ±13.6 years; in controls male/female ratio was 14/24 and the mean age was 38.0+1.41 years. Diabetes mellitus, hypertension, chronic glomerulonephritis were the first three causes of ESRD with percentages of 28, 24, and 23% respectively. Systolic and diastolic blood pressures were higher in patients than controls. ACE gene (l/D) polymorphism prevalence was found as 0.9%, 44.8%, 45.9% among patients, and 31.5%, 39.4%, 28.9% among controls for ll/ID/DD respectively, and statistical significance was found (p=0.006). ACE D allele frequency was 68.4% among patients, and 48.7% among controls (p=0.001). Conclusion: We found difference in dispersion of ACE genotype and frequency of D allele between patients and controls.
Abstract (Original Language): 
Amaç: Diyaliz tedavisi gören hasta popülasyonunda renin an-jiyotensin aldosteron sisteminden, anjîyotensin dönüştürücü enzim gen polimorfizm frekansını değerlendirmektir. Gereç ve Yöntem: Çalışmaya diyaliz tedavisi gören 87 hasta ve kontrol grubu olarak 38 sağlıklı gönüllü alındı. Çalışma kapsamındaki hasta ve kontrol grubunun demografik bilgileri, kan basıncı değerleri, vücut kitle indeksi (VKİ), hematolojik ve biyokimyasal parametreler ve EDTA'lı tüplere alınan periferik kanda steril polimeraz zincir reaksiyonu yöntemi ile incelenen ACE gen polimorfizmi sonuçları kayıt edildi. Bulgular: Çalışmaya alınan hastaların 49'u HD, 38'i SAPD programında idi. Hasta grubunda erkek/kadın 48/39, yaş ortalaması 41.1±13.6 iken, kontrol grubunda 14/24 ve 38.0+1.41 idi. Hasta grubunda SDBY nedenleri olarak %28 diyabet, %24 hipertansiyon, %23 kronik glomerü-lonefrit ilk üç sırayı almaktaydı. Sistolik kan basıncı vediyastolik kan basıncı, hasta grubunda, kontrol grubuna göre daha yüksek bulundu. ACE gen (l/D) polimorfizmi prevalansı hasta grubunda, I l/l D/D D için sırasıyla %0.9, %44.8, %45.9; kontrol grubunda ise %31.5, %39.4, %28.9 bulundu ve istatistiksel farklılık tespit edildi (p-0.006!. Hasta grubu ve kontrol grubu ACE D allel dağılımları açısından hasta grubunda %68.4, kontrol grubunda %48.7 olarak bulundu (p=0.0Ü1). Sonuç: SDBY hasta popülasyonunda ACE genotip dağılımı ve D allel sıklığında kontrol grubundan farklılık tespit ettik.

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