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Dekspantenolün Böbreği İskemi-Reperfüzyon Hasarından Koruyucu Etkisi Var mıdır?

Does Dexpantenol Protect the Kidney from Ischemia-Reperfusion Injury?

Journal Name:

  • Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi

Publication Year:

  • 2011
DOI: 
10.5262/tndt.2011.1002.05

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
ObjectI ves : Tissue injury occurs following reperfusion after creation of ischemia. Plenty of chemical agents have been shown to protect from such an injury. We planned to evaluate the protective effect of dexpanthenol (dxp) in ischemia-reperfusion injury. MaterI al and metho ds: 24 adult rats were used and divided into 3 groups. A right nephrectomy was performed through a median laparotomy incision in all groups. Additionally, in group 1 (sham group), left nephrectomy was made 6 hours later without creation of ischemia. In group 2 (Saline group), the left kidney was left ischemic for 1 hour and reperfusion was established for 6 hours. Saline was administered intraperitoneally thirty minutes before creation of ischemia and just before reperfusion. In group 3 (Dexpanthenol group), the left kidney was left ischemic for 1 hour and reperfusion was established for 6 hours. Dxp (500 mg/kg) was administered intraperitoneally thirty minutes before creation of ischemia and just before reperfusion. A left nephrectomy was performed at the end of the 6 hours of reperfusion. Nephrectomy specimens were histopathologically analysed for congestion, inflammation and necrosis. Tissue NO, glutathione reductase, catalase and MDA levels were measured. Res ults : There was no significant differences between the groups histopathologically or biochemically. ConclusIo n: Dexpanthenol is the biologically active form of pantothenic acid and has an antioxidant effect. Our study was not in correlation with the literature regarding a protective effect of dxp on various organs via its antioxidant effect.
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Abstract (Original Language): 
Amaç: İskemik kalan bir dokuda tekrar reperfüzyon sağlandığında serbest oksijen radikalleri açığa çıkmakta ve iskemiden daha yaygın bir doku hasarı oluşmaktadır. Birçok kimyasal ajanın bu hasarı önlediği bilinmektedir. Çalışmamızda, dekspantenolün (Dxp) böbreği iskemi-reperfüzyon hasarından koruyup korumadığını araştırmayı amaçladık. Gereç ve Yönte mler : Çalışmada 24 erişkin sıçan kullanıldı ve 8’erli 3 grup oluşturuldu. Tüm gruplarda orta hat laparotomi ile sağ nefrektomi yapıldı. Buna ek olarak Grup 1’de (Sham grubu) sol böbrekte iskemi oluşturulmadan 6 saat sonra sol nefrektomi yapıldı. Grup 2’de (Serum fizyolojik grubu) sol böbrek 1 saat iskemik bırakıldı. 1 saatin sonunda 6 saat süre ile reperfüzyon uygulandı. İskemiden 30 dakika önce ve reperfüzyondan hemen önce periton içine serum fizyolojik uygulandı. Grup 3’te (Dekspantenol grubu) sol böbrek 1 saat iskemik bırakıldı ve 6 saat reperfüzyon uygulandı. İskemiden 30 dakika önce ve reperfüzyondan hemen önce 500 mg/kg Dxp periton içine verildi. 6 saatin sonunda sol nefrektomi yapıldı. Alınan her bir böbreğin bir kısmı histopatolojik olarak konjesyon, inflamasyon ve nekroz, bir kısmı da doku nitrik asit, Glutatyon redüktaz, katalaz ve MDA düzeyleri yönünden incelendi. Bulgular: Histopatolojik ve biyokimyasal değerlendirmede gruplar arasında anlamlı bir fark saptanmadı (p>0,05). Sonuç: Dxp, pantotenik asidin biyolojik olarak aktif formudur ve antioksidan etkiye sahiptir. Literatürde çeşitli dokularda antioksidan etkisi ile dokuları koruyucu etkisi gösterilmiş olmasına rağmen bizim çalışmamızda böbreğin iskemi-reperfüzyon hasarında Dxp’ün koruyucu etkisi olduğu gösterilememiştir.
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  • 2
146-149
  • Turkish

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