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KRONİK BÖBREK YETMEZLİĞİNDE DİYALİZ ÖNCESİ VE SONRASI SERUM TOTAL SİYALİK ASİT DÜZEYLERİ

SERUM TOTAL SIALIC ACID LEVELS BEFORE AND AFTER HEMODIALYSIS IN CHRONIC RENAL FAILURE

Journal Name:

  • Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi

Publication Year:

  • 1998

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The aim of this study was to determine the levels of sialic acid in the sera of patients with chronic renal failure and the effect of hemodialysis was also investigated. Regarding this aim, total sialic acid levels (TSA) were studied from the blood samples of 20 patients wiith chronic renal failure and 14 healty volunteers. Serum TSA determinations were carried out by the resorcinol method. In the chronic renal failure group the mean TSA concentration before hemodialysis treatment was 99.81 ± 3.57mg/dl and the mean TSA concentration after hemodialysis treatment was 106.64 ±5.10 mg/dl. The mean TSA level in the control group was 68.73 ± 2.12 mg/dl. The TSA levels before and after hemodialysis treatment both were found to be significantly elevated as compared to control levels (p< 0.001). After hemodialysis treatment the TSA levels were slightly increased than those in before hemodialysis samples, however this increase was not significant statistically.In our study, the serum TSA levels of the chronic renal failure patients that received hemodialysis tretment were significantly higher than those in the healthy group. It has been thought that this increase may be due to the increased synthesis and catabolism of glycoproteins and glycolipids in chronic renal failure patients.
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Abstract (Original Language): 
Bu çalışmada kronik böbrek yetmezliği olan hastalarda serum siyalik asil düzeylerinin belirlenmesi ve hemodiyaliz uygulamalarının serum düzeylerine etkisinin araştırılması amaçlanmıştır. Bu amaçla hemodiyaliz yapılan 20 kronik böbrek yetmezliği hastasından ve 14 sağlıklı gönüllüden alman kan örneklerinde total siyalik asit düzeyleri (TSA) çalışıldı. Serum TSA düzeyi kolorimetrik rezorsinol yöntemiyle ölçüldü. Kronik böbrek yetmezliği hastalarında diyaliz öncesi ortalama TSA düzeyi 99.81± 3.57 mg/dl ve diyaliz sonrası ortalama TSA düzeyi 106.64 ± 5.10 mg/dl idi. Ortalama TSA düzeyi kontrol grubunda ise 68.73 ± 2.12 mg/dl olarak bulundu. Diyaliz öncesi ve diyaliz sonrası TSA düzeylerinin her ikisi de kontrol grubuna göre istatistiksel olarak anlamlı düzeyde yüksek bulundu (p<0.001). Diyaliz sonrası TSA düzeylerinde diyaliz öncesi düzeylerine göre artış oldu; fakat bu artış istatistiksel olarak anlamlı değildi. Çalışmamızda, hemodiyaliz uygulanan kronik böbrek yetmezlikti hastaların serum TSA düzeylerinin, sağlıklı bireylere göre anlamlı düzeyde yüksek olduğu belirlendi. Kronik böbrek yetmezlikti hastalardaki bu artışın glikoprotein ve glikolipidlerin yapım ve yıkımındaki artışa bağlı olabileceği düşünüldü.
FULL TEXT (PDF): 
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  • 4
207-211
  • Turkish

REFERENCES

References: 

1- Waters P.I, Lewry E, Pennock CA. Measurement of sialic acid in serum and urine: clinical application and limitations. Ann Clin Biochem 1992; 29: 625-637.
2- Schauer R. Chemistry, metabolism and biological functions of sialic acids. Adv Carbohydr Chem
7- Blau EB, Hass JE. Glomerular sialic acid and proteinuria in human renal disease. Lab Invest 1973; 28:477-481.
8- Kerjaschki D, Vernillo AT, Farquhar MG. Identification and characterization of podocaiyxin, the major sialoprotein of the renal glomerular epithelial cell. J Cell Biol 1984; 90: 1591-1596.
9- Görög P. Sialic acid moieties on surface glycoproteins protect endothelial cells from proteolytic damage. J Pathol 1985; 146:205-212.
10- Mayes PA. Metabolism of carbohydrate. In: Harper HA,
Rodwell VW and Mayes PA (eds), Review of Physiological Chemistry. Lantz Medical Publication,
California 1979, p:294.
21
Biochem 1982; 40: 131-234.
3- Allalouf D, Gafter U, Malachi T, Hirsh J, Levi J,
Levisky H. Sialic acid and neuraminidase activity in rat kidneys 6 months after uninephrectomy. Biochem Med
MetabBiol 1988; 39: 182-189.
4- Özben T. Elevated serum and urine sialic acid levels in renal diseases. Ann Clin Biochem 1991; 28: 44-48.
5- Svennerholm L. Quantitative estimation of sialic acids. A colorimetric resorcinol-hydrochloric acid method.
Biochim Biophys Acta 1957; 24: 604-611.
6- Bertani T, Poggi A, Pozzoni R, et al. Adriamycin induced nephrotic syndrome in rats: sequence of pathologic events. Lab Invest 1982; 46: 16-23.

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