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İki Farklı Hücre Ayırıcı ile Toplanan Trombositlerin Akım Sitometrik Yöntemle İn Vitro Aktivasyon Markırlarına Etkisinin İncelenmesi

Flow Cytometric Analysis Of In Vitro Activation Markers Of Platelets Collected With Two Different Cell Separators

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Abstract (2. Language): 
The aim of this study was to compare in vitro activation markers of platelets collected with two different cell separators (Cobe Spectra and CS3000 plus) analysed by flow cytometry on day 0 and 3, to determine the relationship between platelet activation and storage time. 5O donor (5 women 45 men) aged between 20-49 (median age 33.4±8.7) who were good candidate for apheresis and who admitted to apheresis for the first time were selected for the study. The platelet activation markers of collections were assessed on day 0 and on day 3 by flow cytometry. The 0. day analysis was performed without time delay as soon as the concentrate was collected and 3. day analysis was performed on the product which was stored on ajitator. CD62 and CD63 expression percentage were high on the third day of storage in both collections of two cell separators Cobe Spectra and CS3000 plus.
Abstract (Original Language): 
Bu çalışmanın amacı, iki farklı hücre ayırıcı Cobe Spectra ve CS3000 Plus cihazlarıyla toplanan trombosit süspansiyonlarının optimal şartlarda saklanarak, 0. ve 3. günlerdeki in vitro aktivasyon markırlarını (trombosit membran yüzey glikoprotein ekspresyonları) akım sitometrik yöntemle bakarak, değişik cihazlarda hazırlanan trombosit süspansiyonlarını karşılaştırmak, saklama zamanı ile trombosit aktivasyonu arasındaki ilişkiyi saptamaktır. Çalışmamız, Aferez Ünitesi’ne başvuran, aferez şartlarını taşıyan, ilk kez trombosit donörü olarak işleme alınan, yaşları 20-49 arasında değişen 5’i kadın, 45’i erkek (yaş ortalaması 33.4±8.7) olmak üzere, toplam 50 donör ile gerçekleştirildi. Örneklerden 0. günde bekletmeden oda ısısında, ajitatörde bekleterek 3. günde olmak üzere iki kez, akım sitometrik yöntemle in vitro trombosit aktivasyon markırları değerlendirildi. İki farklı hücre ayırıcıda da depolamanın 3. gününde CD62 ve CD63 ekspresyonu yüzdesi yüksek bulundu.
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