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Streptozotosinle Oluşturulan Diabetin Sıçan Periferik Sinirleri Üzerine Etkisinin: Elektron Mikroskobik İncelenmesi

An Ultrastructural Study of Effects of Streptozotocin Induced Diabetes on Rats Peripheral Nerves

Journal Name:

  • Uludağ Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2006

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
In this study, structural changes caused by acute effects of STZ induced diabetes on peripheral nerves were investigated with electron microscope. Twenty four male Wistar Albino rats were divided into 4 groups: control, sham, 15 days and 30 days diabetic groups. Nothing applied to control group; sodium citrate, as STZ solvent, to Sham group and 45 mg/kg STZ solved in sodium citrate to diabetic group. 15 and 30 days after injections, rats were sacrified under high dose anesthesia, and sciatic nerves were extracted for electron microscopic examination. A evident gap between perineurium and nerve fibres were observed in both 15 and 30 days diabetic groups. And in 30 days group, myelin sheath degenerations were more obvious in myelinated nerve fibres. On the other hand, Schwann cells and unmyelinated nerve fibres appeared normal. Consequently, STZ induced diabetes were seen to cause degenerative changes in n. ischiadicus and those changes were more evident in 30 days diabetic group.
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Abstract (Original Language): 
Bu çalışmada sıçanlarda streptozotosinle (STZ) oluşturulan diabetin akut etkisinin periferik sinir üzerinde oluşturduğu yapısal değişiklikler elektron mikroskop düzeyinde incelenmiştir. Çalışmada denekler, 24 erkek Wistar albino sıçan, kontrol grubu, Sham grubu, 15 ve 30 günlük diabet grubu olacak şekilde dört grup olarak ayrılmıştır. Kontrol grubuna hiçbir şey uygulanmazken, Sham grubuna STZ’nin çözündüğü sodyumsitrat, diabetik gruba ise sodyumsitrat içinde çözünmüş 45mg/kg STZ uygulanmıştır. Enjeksiyonu takiben 15 ve 30. günlerde sıçanlar yüksek doz anestezi altında sakrifiye edildi ve siyatik sinirlerinin medial bölgeleri çıkarılarak rutin elektron mikroskop takibine alındı. Doku kesitleri incelendiğinde hem 15 hem de 30 günlük diabetik grupta perinöryum ile sinir lifleri arasında belirgin bir açıklık meydana gelmiş ve miyelinli sinir liflerinde miyelin kılıf dejenerasyonları 30 günlük grupta daha belirgin bulunmuştur. Buna karşın, Schwann hücreleri ve miyelinsiz sinir lifleri normal yapıda gözlenmiştir. Sonuç olarak bu çalışmada sıçanlarda STZ ile oluşturulan diabet, n.ischiadicus’ta dejeneratanif değişiklikler oluşturmuş ve bu değişiklikler 30 günlük grupta daha belirgin bulunmuştur.
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  • 3
77-81
  • Turkish

REFERENCES

References: 

1. Mohseni S, Hildebrand C. Hypoglycaemnic neuropathy in
BB/W or rats treated with insulin implans: Electron
microscopic observations. Acta neuropathol (Berl) 1998;
96:151-56. Streptozotosinle Diabetin Sıçan Periferik Sinirleri Üzerine Etkisi
81
2. Baynes JW. Role of oxidative stres in development of
complications in diabetes. Diabetes 1991; 40: 405-12.
3. Nickander K.K, Schmelzer JD, Rohwer DA, Low P.A. Effect
of alphatocopherol deficiency on induces of oxidative stres in
normal and diabetic peripheral nerve. J Neorol Sci. 1994; 126:
6-14.
4. Van dam PS, Bravenboer B, Van asbeck BS, Van oırschot
JFLM, Marx JJM, Gispen WH. Effect of insulin treatment on
endoneurial and systemic oxidative stres in relation to nerve
conduction in streptozotocin-diabetic rats. Eur J Clin İnvest
1996; 26: 1143-49.
5. Sharma AK, Thomas PK, and DeMolina AF. Peripheral nerve
fibre size in experimental diabetes. Diabetes 1977 ; 26: 689-92.
6. Sharma AK, Thomas PK, Gabriel G, Stoliski C, Dockeri P,
Hollins GV. Peripheral nerve abnormalities in the diabetic
mutant mouse. Diabetes 1983; 32: 1152-61.
7. Jakobsen J, Lundbeak K. Neuropathy in experimental diabetes.
An animal model. Br. Med J 1976; 2: 278-79.
8. Jacobsen J. Axonal dwindling in early experimental diabetes, I.
A study of cross- sectioned nerves. Diabetologia 1976; 12:
539-46.
9. Jacobsen J. Early and preventable chances of peripheral nerve
structure and function in insulin deficient diabetic rats. J.
Neurol. Neurosurg. Psychiat 1979; 42: 509-18.
10. Sharma AK, Bajada S, Thomas PK. İnfluence of
streptozotocin-induced diabetes on myelinated nerve fibre
maturation and on body growth in the rat. Acta Neuropathol.
(Berl.) 1981;53:257-65.
11. Dockery P, Sharma AK. Ultrastructural abnormalities of
miyelinated fibres in the tibial nerve of streptozotocin-diabetic
rats. J Neurol Sci. 1990;98: 327-45.
12. Yagihashi S, Kamijo M, Watanabe K. Reduced myelinated
fiber size correlates with loss of axonal neurofilaments in
peripheral nerve of chronically streptozocotin diabetic rats.
Am J pathol 1990; 136: 1365-73.
13. Chopra JS, Sawhney BB, Chakravorty RN. Pathology and
time relationship of peripheral nerve changes in experimental
diabetes. J Neurol Sci 1977; 32: 53-67.
14. Kennedy WR, Quick DC, Miyoshi T, Geritsen GC. Peripheral
neurology of the diabetic Chinese hamster. Diabetologia 1982;
23: 445-52.
15. Sharma AK, Thomas PK, Gabriel G, Stolinski C, Dockery P,
Hollins GW. Peripheral nerve abnormalities in the diabetic
mutant Mouse. Diabetes1983:32: 1152-61.
16. Walker D, Malık RA, Anderson H, Gardiner TA., Boulton
AJM. The effects of Sulindac on peripheral nerve morphology
in the chemically induced diabetic dog. Diabetologia 1997; 40
(suppl.1): A550.
17. Walker D, Carrington A, Canan C.A, Sawıckı D, Sredy J,
Boulton A.J.M. and Malık, R.A. Structural abnormalities do
not explain the early functional abnormalities in the prepheral
nerves of the streptozotocin diabetic rat. J. Anat, 1999;95:
419-27.
18. Sharma AK, Malık RA, Dhar P, Mehra RD, Ahmed I, Lowrie
CT. Peripheral nerve abnormalities in spontaneously diabetic
dog.. İnt J Diabetes 1995; 3: 130-39
19. Britland ST, Young RJ, Sharma AK, Clarke BF. Association
of painful and painless diabetic polyneuropathy with different
patterns of nerve fibre degeneration and regeneration.
Diabetes 1990; 39 :898-908.
20. Llewelyn JG, Gilby SG, Thomas PK, Kıng RHM, Muddle JR,
Watkins PJ. Sural nerve morphometry in diabetic autonomic
and painful sensory neuropathy. Brain 1990; 114: 867-92.
21. Malık RA. The pathology of human diabetic neuropathy.
Diabetes 1997; 46: 50-3.
22. Ferreira LD, Huey PU, Pulford BE, İshii DN, Eckel RH.
Sciatic nerve hipoprotein lipase is reduced in streptozotocininduced diabetes and corrected by insulin. Endocrinology
2002; 143(4): 1213-7.
23. Giannini C, Dyck PJ. Basement membrane reduplication and
pericyte degeneration precede development of diabetic
polyneuropathy anda re associated with its severity. Ann
Neurol 1995; 37: 498-504.
24. Malik RA, Newrick PG, Sharma AK., Jennings A, Ah-see AK,
Mayhew TM. Microangiopathy in human diabetic neuropathy:
relationship between capillary abnormalities and the severity
of neuropathy. Diabetologia 1989; 32: 92-102.
25. Khara M, Zollman PJ, Smithson IL, Lagerlund TD, Low PA.
Hypoxic effect of exogenous insulin on normal and diabetic
peripheral nerve. Am J Physiol 1994; 266: 980-85.
26. Sharma AK, Thomas PK. Peripheral nerve structure and
function in experimental diabetes. J Neurol Sci 1974; 23: 1-15.
27. Brown MJ, Sumner AJ, Greene DA, Diamond SM., Asbury
AK. Distal neuropathy in experimental diabetes mellitus. Ann
Neurol 1980; 8: 168-78.
28. Sugimura K, Windebank AJ, Natarajan V, Lambert EH,
Schmid H, Dyck PJ. Interstitial hyperosmolarity may cause
axis cylinder shrinkage in streptozotocin diabetic nerve. J
Neuropath and Exp Neur 1980; 39: 710-21.
29. Quasthoff S.: The role of axonal ion conduntances in diabetic
neuropathy: a review. Muscle & Nerve 1998; 21: 1246-55.
30. Boulton AJM, Malik RA. Diabetic neuropathy. Med Clin N
Am 1998; 82: 909-29.

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