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Prolin Uygulamasının Defne Fidelerinin Kuraklık Toleransının Uyarılması Üzerine Etkileri

Effects of Proline Treatment on Inducing Drought Tolerance of Laurel Seedlings

Journal Name:

  • Cumhuriyet Üniversitesi Fen Fakültesi Fen Bilimleri Dergisi

Publication Year:

  • 2015

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The effects of proline treatment on inducing drought tolerance and phytohormone content of Mediterranean evergreen sclerophyll Laurus nobilis L. (laurel) were studied in semi-controlled greenhouse conditions. Half of the one-month-old seedlings were subjected to water deficit (25 % of field capacity) during 5 months and other half was irrigated regularly at field capacity and served as controls. Both groups treated with proline. While leaf osmotic potential reached to -1.5 MPa in control group, proline treatment under drought stress led to more negative values (-2.53 MPa) in seedlings. An enhancement in the photochemical efficiency of PSII, maintaining fresh weight and relative water content and increase in survival capacity of seedlings (%52) were recorded by the effect of proline treatment under drought stress conditions. The phytohormones indole-3-acetic acid (IAA), zeatin (Z) and giberellic acid (GA3) contents of leaves were lower as compared to control ones, while abscisic acid (ABA) was higher under water stress conditions. Under same conditions only IAA content was increased but Z and GA3 contents were not changed by proline treatment. Although drought conditions induced ABA accumulation in leaves proline treatment suppressed high level ABA accumulation under same conditions. The data indicate that proline treatment in seedling stage may induce drought tolerance of laurel plants.
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Abstract (Original Language): 
Akdeniz her dem yeşil sklerofillerinden Laurus nobilis L. (defne) fidelerinde prolin uygulamasının, kuraklık toleransının uyarılması ve hormon içeriği üzerindeki etkileri yarı-kontrollü sera koşullarında çalışılmıştır. Bir aylık fidelerin yarısı kontrol grubu olarak tarla kapasitesinde sulanmış, diğer yarısı ise 5 ay boyunca su eksikliğine (tarla kapasitesinin %25‘i) maruz bırakılmış ve her iki gruba da dışarıdan prolin uygulanmıştır. Yaprak ozmotik potansiyeli kontrol grubu fidelerinde -1.5 MPa değerlerinde iken, su stresi koşullarında prolin uygulaması ile daha negatif ozmotik potansiyel değerleri (-2.53 MPa) ölçülmüştür. Su stresi koşullarında fidelere prolin uygulaması ile yaprak PSII fotokimyasal etkinliğinin arttığı, taze ağırlık ve bağıl su içeriğinin korunduğu ve fidelerin hayatta kalma oranının (%52) yükseldiği belirlenmiştir. Su stresi, defne fidelerinde içsel indol-3-asetik asit (IAA), zeatin (Z) ve giberellik asit (GA3) içeriğinde azalmaya, absisik asit (ABA) içeriğinde ise artışa neden olmuştur. Su stresi koşullarında fidelere yapılan prolin uygulanmasının, yalnız IAA içeriğinde artışa neden olduğu, fakat Z ve GA3 içeriğinde kuraklık etkisi ile oluşan azalmayı değiştirmediği belirlenmiştir. Su stresi koşullarının ABA birikimini uyardığı fakat aynı koşullar altında fidelere yapılan prolin uygulanmasının ABA miktarındaki artışı baskıladığı belirlenmiştir. Elde edilen veriler, defnenin fide döneminde prolin uygulaması ile kuraklığa toleransını uyarılabildiğini ortaya koymaktadır.
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