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Effect of temperature and serum–clot contact time on the clinical chemistry laboratory results

Journal Name:

  • Indian Journal of Basic & Applied Medical Research

Publication Year:

  • 2014

Key Words:

Author Name
Abstract (2. Language): 
Introduction- Clot contact time and temperature have variable effect on the stability of laboratory investigations. This study is conducted to establish the maximum time delay acceptable between sample collection and separation of serum and the optimum temperature which should be maintained during this time delay. Methods and materials- six morning samples from 30 healthy volunteers are collected.3 samples from each volunteer is stored for 0hrs, 3hrs and 6hrs at 230C and another 3 samples at 0 hrs, 3hrs and 6hrs at 320C. stability of electrolytes is analyzed by repeated measure anova. Results and discussion- stability of electrolytes (sodium and potassium) is not altered when samples are stored at 230C. The maximum clot contact times which have no effect the stability of electrolytes is 3hrs.
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REFERENCES

References: 

1) Tuck MK, Chan DW, Chia D, Godwin AK,Grizzle WE,Krueger KE et al. Standard Operating Procedures for Serum
and Plasma Collection: Early Detection Research Network Consensus Statement Standard Operating Procedure
Integration Working Group.2010. J Proteome Res; 8: 113-17
2) Stankovic AK. The laboratory is a key partner in assuring patient safety. Clin Lab Med 2004;24:1023-35).
Indian Journal of Basic and Applied Medical Research; December 2014: Vol.-4, Issue- 1, P. 356-362
www.ijbamr.com P ISSN: 2250-284X , E ISSN : 2250-2858
362
3) Burtis CA, Ashwood ER, eds. Tietz textbook of clinical chemistry, 2nd ed.Philadelphia, PA: WB Saunders, 1994:76
4) Laessig RH, Indriksons AA, Hassemer DJ, Paskey TA, Schwartz TH. Changes in serum chemical values as a result of
prolonged contact with the clot. Am J Clin Path 1976;66:598–604.
5) Ono T, Kitabuchi K, Takehara M, Shiiba M, Hayami K. Serum constituents analyses: effect of duration and temperature of
storage of clotted blood. Clin Chem 1981;27:35–8.
6) Chu SY, MacLeod J. Effect of three-day clot contact on results of common biochemical tests with serum. Clin Chem 1986;32:
2100.
7) Rehak NN, Chiang BT. Storage of whole blood: effect of temperature on the measured concentration of analytes in serum.
Clin Chem 1988;34:2111–4.
8) Heins M, Heil W, Withold W. Storage of serum or whole blood samples? Effects of time and temperature on 22 serum
analytes. Eur J Clin Chem Biochem 1995;33:231–8.
9) Burtis CA, Ashwood ER, Bruns DE. Siggard –Anderson O. Electrochemistry, Tietz textbook of chemical chemistry.
Philadelphia; WB saunders,1986;110-125)
10) ISO 5725-6. Accuracy (trueness and precision) of measurement methods and results.Part 6: use in practice of accuracy values;
1994.
11) Burtis CA Ashwood ER eds. Tietz textbook of clinical chemistry , 2nd ed.Philadelphia, PA: WB Saunders, 1994:82
12) Young DS, Bermes EW. Specimen collection and processing : sources of biological variation.In:Tietz NW,ed.Textbook of
clinical chemistry, Philadelphia : WB Saunders Co.,1986:478-518.
13) Danowski TS. The transfer of potassium across the human blood cell membrane. J Biol Chem 1941;139:693-705.
14) Goodman JR, Vincent J, Rosen I. Serum potassium changes in blood clots. Am J Clin Pathol 1954;24:111-113.
15) Zhang DJ, Elswik RK, Miller WG, Bailey JL.Effect of serum clot contact time on clinical chemistry laboratory results.Am J
Clin Chemistry 1998;44:1325-1333.

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