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Effect of Adding Fatty Acid in Culture Medium on Cell Growth of Acid Tolerant Lactic Acid Bacterium

Journal Name:

  • International Journal of Science and Engineering Investigations

Publication Year:

  • 2017

Key Words:

Author NameUniversity of Author
Abstract (2. Language): 
Tween 80 is important growth factor for lactic acid bacterium, especially for Lactobacillus spp. The composition of fatty acids of the lipid in cell membrane of highly acid-tolerant Lactobacillus acetotolerans HT was determined and the effect of tween 80 and 9 kinds of fatty acid on the cell growth was investigated. The strain HT grew after very long lag phase for about 300h in synthetic MRS medium without addition of fatty acid. Tween 80, stearic acid, oleic acid or cis-vaccenic acid stimulated the growth of strain HT. Particularly, the lag phase was drastically shortened by addition of 5mg/l of oleic acid or cis-vaccenic acid as well as 1000mg/l of tween 80. However, the addition of oleic or cis-vaccenic acid more than 50mg/l suppressed seriously the cell growth. Other kinds of unsaturated C18 (linoleic acid, conjugated linoleic acid, elaidic acid) and cycC19:0⊿11 (lactobacillic acid) were also effective for the enough growth of the strain HT. These results indicate that fatty acid is not essential for the growth of Lb.acetotolerans HT. However, fatty acid is very important to shorten the long lag phase of this bacterium and the effect greatly differs depending on kind of fatty acid and the concentration.
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REFERENCES

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