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Yerel Tunus arpa arpa genetik çeşitlilik genetik çeşitlilik, RAPD ve SSR analizi dayalı

Genetic diversity in barley genetic diversity in local Tunisian barley based on RAPD and SSR analysis

Journal Name:

  • Biyolojij Çeşitlilik ve Koruma

Publication Year:

  • 2009

Key Words:

Author Name
Abstract (2. Language): 
The genetic variation and relationships among 12 local barleys and the varieties Martin and Manel were evaluated using random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR). A high level of polymorphism was found with both RAPD and SSR markers and the mean polymorphism information content (PIC) values were 0.477 and 0.533 for RAPD and SSR markers, respectively. In RAPD analyses, 69 out of 93 bands (74%) were polymorphic. The number of alleles ranged from 4 to 10 per primer, with an average of 6.2 per primer. The RAPD-based genetic dissimilarity (RAPD-GD) ranged from 0.114 to 0.933, with the mean of 0.523. In SSR analyses, a total of 43 alleles were detected, among which 39 alleles (90.7%) were polymorphic. The number of alleles per primer ranged from 2 to 4 with an average of 2.87 alleles per SSR primer. The SSR-derived genetic dissimilarity (SSR-GD) ranged from 0.423 to 0.910, with the mean of 0.665. SSR was better than RAPD to detect genetic diversity among the barley accessions. A poor correlation (r = 0.193) was found between both sets of genetic similarity data, suggesting that both sets of markers revealed unrelated estimates of genetic relationships.
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REFERENCES

References: 

Akkaya MS, Bhagwast AA, Cregan PB .1992. Length polymorphisms of simple sequence repeat DNA in soybean. Genetics. 132. 1131-1139.
Bahattin T .2003. Inter-simple sequence repeat (ISSR) and RAPD variation among wild barley (Hordeum vulgare
subsp. spontaneum) populations from west Turkey. Genetic Resources and Crop Evolution. 50. 611-614. Baird WV, Ballard RE, Rajapkse S, Abbott AG .1996. Progress in Prunus mapping and application of molecular
markers to germoplasm improvement. Hort. Sci. 31. 1099 - 1106. Barua UM, Chalmers KJ, Hackett CA, Thomas WTB, Powell W, Waugh R .1993. identification of RAPD markers
linked to a phynchosporium secalis resistance locus in barley using nearisogenic lines and bulked segregant
analysis. Heredity.71. 177-184. Ben Hmida SW (2000). Contribution des marqueurs microsatellites et RAPD pour l'etude du polymorphisme chez
l'orge. Diplome d'etude approfondie de genetique et biologie moleculaire. Fac.sci.Tunis. Bernard RB, Nevo E, Douglas AJ, Beiles A .1997. Genetic diversity in wild barley (Hordeum spontaneum C. Koch) in
the near east: a molecular analysis using random amplified polymorphic DNA (RAPD) markers. Genetic
Resources and Crop Evolution. 44. 147-157. Bustos AD, Casanova C, Soler C, Jouve N .1998. RAPD variation in wild populations of four species of the genus
Hordeum (Poaceae). Theor Appl Genet. 96. 101-111. Botstein D, White RL, Skolnick M, Davis RW .1980. Construction of a genetic linkage map in man using restriction
fragment length polymorphisms. Am. J. Hum. Genet. 32. 314-331. Chen XP, Yan L, Ding Y, Gao WX .2000. Analysis of germplasms of Hubei barley by randomly amplified polymorphic
DNA
(RAPD)
. J. Wuhan Univ. (Natural Science Edition). 46. 249-252. Chen XP, L Yan, Y Ding. 2000b. RAPD analysis and the probable evolutionary route of wild relatives of barley from
China. Acta Botanica Sinica. 42. 179-183. Cheng ZP, Huang HW (2004). Genetic diversity and genetic structure of different groups in Prunus persica reveleted by
RAPD analysis.J. Wuhan Bot. Res. 22. 27-32 (in Chinese with an English abstract). Chalmers KJ, Barua UM, Hackett CA, Thomas WTB, Waugh R, Powell W .1993. Identification of RAPD markers
linked to genetic factors controlling the milling energy requirement of barley. Theor Appl Genet. 87. 314-320. Davila JA, Loarce Y, Ramsay L, Waugh R, Ferrer E .1999a. Comparison of RAMP and SSR markers for the study of
wild barley genetic diversity. Hereditas. 131. 5-13. Davila JA, Loarce Y, Ferrer E .1999b. Molecular characterization and genetic mapping of random amplified
microsatellite polymorphism in barley. Theor Appl Genet. 98. 265-273.
K. Karim, at al., Genetic diversity in barley Genetic diversity in local Tunisian barley based on RAPD and SSR analysis
34 Biological Diversity and Conservation - 2 / 1 (2009)
Davila JA, Sanchez MP , Loarce Y, Ferrer E .1998. The use of random amplified microsatellite polymorphic DNA and
coefficients of parentage to determine genetic relationship in barley. Genome. 41. 477- 486. Devos KM, Gale MD .1992. The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet. 84.
567-572.
Feng ZY, Zhang YZ, Zhang LL, Ling HQ .2003. Genetic diversity and geographical differentiation of Hordeum vulgare ssp. spontaneum in Tibet using microsatellite markers. High Technology Letters.10. 46-53.
Fernandez ME, Figueiras AM, Benito C .2002. The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theor Appl Genet. 104.
845-851.
Joshi Cp, Nguyen HT .1993. RAPD (Random amplified polymorphic DNA) analysis based on intervarietal genetic
relationships among hexaploid wheats. Plant Sci. 93. 95-103. Karp A, Edwards K, Bruford M .1997. Newer molecular technologies for biodiversity evaluation: opportunities and
challenges. Nature Biotechnol. 15. 625-628. Kantety RV, Zeng XP, Bennetzen JL, Zehr BE .1995. Assessment of genetic diversity in Dent and Popcom (Zea mays
L.) inbred lines using inter-simple sequences repeat (ISSR) amplification. Mol Breed. 1. 365-373. Konishi T .2001. Genetic diversity in Hordeum agriocrithon E. Aberg, six-rowed barley with brittle rachis, from Tibet.
Genetic Resource and Crop Evolution. 48. 27-34. Loarce Y, Gallego R, Ferrer E .1996. A comparative analysis of genetic relationship between rye cultivars using RFLP
and RAPD markers. Euphytica. 88. 107-115.
Liu F, Sun GL, Salomon B, Bothmer R .2002. Characterization of genetic diversity in core collection accessions of wild
barley, Hordeum vulgare ssp. spontaneum. Hereditas. 136. 67-73. Matus IA, Hayes PM .2002. Genetic diversity in three groups of barley germplasm assessed by simple sequence repeats.
Genome. 45. 1095-1106.
Mantel M .1967. The detection of disease clustering and a generalized regression approach. Cancer. Res. 27. 209-220. Metais I, Aubry C, Hamon B, Jalouzot R .2000. Description and analysis of genetic diversity between commercial bean
lines (Phaseolus vulgaris L.). Theor Appl Genet. 101. 1207-1214. Nevo E, Zohary D, Brown AHD, Haber M .1979. Genetic diversity and environmental associations of wild barley,
Hordeum spontaneum, in Israel. Evolution. 33. 815-833. Nevo E, Zohary D, Beiles A, Kaplan D, Storch N .1986. Genetic diversity and environmental associations of wild
barley, Hordeum spontaneum, in Turkey. Genetica. 68. 203-213. Nagaoka T, Ogihara Y .1997. Applicability of inter-simple sequence repeat polymorphism in wheat for use as DNA
markers in comparison to RFLP and RAPD markers. Theor Appl Genet. 94. 597-602. Nei M, Li W .1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc.
Natl. Acad. USA. 76. 5269-5273.
Plucknett DL, Smith NJH, Williams JT, Murthi AN .1987. Genebanks and the world's food. Princeton University Press, Princeton New Jersey, USA.
Powell W, Morgante M, Andre C .1996. The comparision of RFLP, RAPD, AFLP and SSR(microsatellite) marker for
germplasm analysis. Mol Breeding. 2. 225-238. Qian W, Ge S, Houng DY .2001. Genetic variation within and among populations of a wild rice Oryza granulata from
China detected by RAPD and ISSR markers. Thore Appl Genet. 102. 440-449. Rohlf FJ .1993. NTSYS-pc version 1.80. Distribution by Exeter Software, Setauket, New York.
Russell JR, Fuller JD, Macaulay M, Hatz BG, Jahoor A, Powell W, Waugh R .1997. Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor Appl Genet. 95.
714-722.
Sokal RR, Rohlf FJ .1995. Biometry: The principles and practice of statistics in biological research. (3d edition). WH
Freeman and Company. New York. USA. Saiki RK, Gelfond DH, Stoffel S, Scharf SJ, Higuchi R, Horn BT, Mullis KB, Erlich HA .1988. Primer-directed
anzymatic amplification of DNA with a thermostable DNA polymerase. Science. 239. 487-491. Sanchez de la Hoz MP, Davila JA, Loarce Y, Ferrer E .1996. Simple sequence repeat primers used in polymerase chain
reaction amplifications to study genetic diversity in barley. Genome. 39. 112-117. Sosinski B, Gannavarapu M, Hager LD, Beck LE, King GJ, Ryder CD et al. .2000. Characterization of microsatellite
markers in peach (Prunuspersica (L.) Batsch). Thor.Appl.Genet. 101. 421-428. Struss D, Plieske J .1998. The use of microsatellite markers for detection of genetic diversity in barley populations.
Theor Appl Genet. 97. 308-315.
Shi YT, Bian HW, Han N, Pan JW, Tong WX, Zhu MY .2004. Genetic variation analysis by RAPD of some barley
cultivars in China. Acta Agronomica Sinica. 30/3. 258-265. Sneath PHA, Sokal RR .1973. Numerical Taxonomy. Freeman, San Francisco.
Tragoonrung S, Kanazin V, Hayes PM, Blake TK (1992). Sequence-tagged-site facilitated PCR for barley genome mapping. Theor Appl Genet. 84. 1002-1008.
K. Karim, at al., Genetic diversity in barley Genetic diversity in local Tunisian barley based on RAPD and SSR analysis
Biological Diversity and Conservation - 2 / 1 (2009)
35
Tanyolac B .2003. Inter-simple sequence repeat (ISSR) and RAPD variation among wild barley (Hordeum vulgare subsp. spontaneum) populations from west Turkey. Genetic Resources and Crop Evolution. 50. 611-614.
Tian HL, Yin XL, Zi CL, Hong LZ, Yong WQ, Tao W .2008. Simple sequence rpeat analysis of genetic diversity in primary core collection of peach (Prunuspersica). J.Integ.Plant.Biology. 50, 102-110.
Tinker NA, Fortin MG, Mather DE .1993. Random amplified polymorphic DNA and pedigree relationship in spring
barley. Theor Appl Genet. 85. 976-984.
Von Bothmer R, Jacobsen N, Baden C, Jorgensen RB, Linde-Laursen I .1995. An ecogeographical study of the genus Hordeum. International Plant Genetic Resources. Rome, P.29.
Welsh J, McClelland J .1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18. 7213¬7218.
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV .1990. DNA polymorphisms amplified by arbitrary
primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535. Wu KS, Rones J, Danneberger L .1994. Detection of microsatellite polymorphisms without cloning. Nucleic Acids Res.
22. 3257-3258.
Wu W , Zheng YL , Chen L , Wei YM , Yang RW, Yan ZH .2005. Evaluation of genetic relationships in the genus Houttuynia Thunb. in China based on RAPD and ISSR markers. Biochemical Systematics and Ecology. (Accepted).
Yang XG, Zhang KC, Qin L, Wang YX .2001. RAPD analysis of germpolasm resources on peach. J.fruit Sci. 18. 276¬279 (in Chinese with an English abstract). Zietkiewicz E, Rafalski A, Labuda D. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics.20. 176-783.

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