COMPARATIVE STUDY OF NEW TRENDS IN HPLC

This article represents a brief review of new trends in HPLC along with its principle and instrumentation. It describes about comparative study of new trends in HPLC such as RRLC, UPLC, UFLC & Nano LC. Mainly we discuss in terms of instrumental operating conditions such as column temperature, flow rate, injection volume and also cover the applications and advantages over HPLC of each technique. Superiority of modern instruments consists of internal diameter reduction, increases sensitivity and/or less sample requirement, significantly cheaper, quicker than its conventional counterpart and increased detection sensitivity in MS because of lower flow rates in smaller columns. Recent developments have significantly increased the resolution power for complex sample analysis.