You are here

Home » Content

İNTRAPERİTONEALMELATONİN ENJEKSİYONU VE PİNEALEKTOMİNİN DENEYSEL AKUT PANKREATİTE ETKİSİ

THE EFFECTS OF INTRAPERITONEAL MELATONIN INJECTION AND PINEALECTOMY IN EXPERIMENTALACUTE PANCREATITIS

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2009

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objectives: In this study the aim was to evaluate the effects of on biochemical markers and histopathological findings of pancreas tissue of decreeasing and increasing serum melatonin levels in rats with had experimental acute pancreatitis. Materials and methods: This study was conducted three groups of Winstar Albino rats (ten animals in every group) that have experimental acute pancreatitis. First group was evaluated as control group. Surgical pinealectomy was applied in the second group and the source of endogen melatonin was (low melatonin levels group). Third group of rats injected with 0.1 ml daily intraperitoneal injection of 20 mg/ml melatonin solution for one week was high melatonin levels group. The effects of melatonin levels on acute pancreatitis were evaluated by comparing these group according to histopathologial results of polimorphonuclear leukocytes. infiltration, oedema, haemorrhagie, apoptosis, aciner cellular degeneration in pancreatic tissue and the levels of malonyledialdeyde (MDA), glutathione reductase (GSH), superoxyde dismutase (SOD), aspartate transaminase (AST), alanine transpherase (ALT), amylase, lactate dehydrogenase (LDH) and bilirubine. Results:We investigated that high melatonin levels has an decreasing effect on of polimorphonuclear leukocyte infiltration, oedema, haemorrhage, aciner cellular degeneration, apoptosis in pancreatic tissue, AST, LDH, bilirubine and MDA levels. Conclusion: As a conclusion exogenous melatonin may have a preventive effect on inflammatory process by reducing oxidative damage in pancreatic tissue.
Bookmark/Search this post with
Abstract (Original Language): 
Amaç: Bu çal›flmada deneysel olarak akut pankreatit oluflturulan ratlarda, kandaki melatonin düzeyinin azalt›lmas› veya artt›r›lmas›n›n pankreas dokusunda neden oldu¤u histopatolojik de¤ifliklikler ve baz› biyokimyasal belirteçlere etkisinin de¤erlendirilmesini amaçlad›k. Gereç ve yöntem: Bu çal›flma deneysel akut pankreatit oluflturulan ve her grupta 10 hayvan bulunan 3 grup Winstar Albino cinsi s›çan üzerinde yürütüldü. Birinci grup denekler kontrol grubu olarak de¤erlendirildi. ‹kinci grup deneklerde pinealektomi yap›larak temel endojen melatonin kayna¤› ortadan kald›r›ld› (düflük melatonin düzeyli grup). Bir hafta boyunca günde 20 mg/ml melatonin solusyonundan 0,1 ml intraperitoneal enjeksiyon yap›lan üçüncü gruptaki ratlar ise yüksek melatonin düzeyli grubu oluflturmufltur. Bu gruplar akut pankreatitin biyokimyasal parametreleri ve histopatolojik bulgular› aç›s›ndan karfl›laflt›r›larak melatonin düzeyinin akut pankreatite etkisi araflt›- r›lm›flt›r. Gruplar pankreas dokusunda polimorf nükleer lökosit infiltrasyonu, ödem, hemoraji, asiner hücre dejenerasyonu ve apoptozis gibi histopatolojik de¤ifliklikler, malonildialdehid (MDA), glutatyon (GSH) ve superoksid dismutaz (SOD) düzeyleri, biyokimyasal olarak serum aspartat transaminaz (AST), alanin transferaz (ALT), amilaz, laktat dehidrogenaz (LDH) ve bilirubin de¤erlerindeki de¤ifliklikler aç›s›ndan karfl›laflt›r›ld›. Bulgular: Yüksek melatonin düzeyinin pankreas dokusunda polimorf nükleer hücre infiltrasyonunu, ödem, hemoraji, asiner hücre dejenerasyonu, apoptozi, AST, LDH, amilaz, bilirubin ve doku MDA düzeyini azalt›c› etkisi oldu¤ u tespit edilmifltir. Sonuç: Sonuç olarak ekzojen melatonin verilmesinin, pankreasta oksidatif hasar› azaltmak suretiyle dokudaki inflamatuar reaksiyonlar› k›smen önleyici bir etkiye sahip oldu¤u kanaatine var›lm›flt›r.
FULL TEXT (PDF): 
PDF icon arastirmax-intraperitonealmelatonin-enjeksiyonu-pinealektominin-deneysel-akut-pankreatite-etkisi.pdf
  • 3
84-92
  • Turkish

REFERENCES

References: 

1. Alhan E, Kalyoncu NI, Kural BV, Erçin C. Effects of melatonin
on acute necrotizing pancreatitis in rats. Z Gastroenterol. 2004;
42:967-972.
2. Banks PA, Freeman ML: Practice Parameters Committee of the
American College of Gastroentrlogy. Practice guidelines in acute
pancreatitis. Am J Gastroenterol. 2006; 101:2379-2400.
3. Beger HG, Rau BM. Severe acute pancreatitis: Clinical course
and management. World J Gastroenterol. 2007; 13:5043-5051.
Melatonine in acute pancreatitis
‹stanbul T›p Fakültesi Dergisi Cilt / Volume: 72 • Say› / Number: 3 • Y›l/Year: 2009
- 91 -
4. Beutler E. Red Blood cell metabolism: Amanual of biochemical
methods. In Beutler E, Ed. 2 nd Ed. New York: Grune&Stratton
Inc, 1984; pp74-76.
5. Bhatia M. Apoptosis versus necrosis in acute pancreatitis. Am J
Physiol Gastrointest Liver Physiol. 2004; 286:G189-196
6. Brzozowski T, Zwirska-Korczala K, Konturek PC, Konturek SJ,
Sliwowski Z, Pawlik M, Kwiecien S, Drozdowicz D, Mazurkiewicz-
Janik M, Bielanski W, Pawlik WW. Role of circadian
rhythm and endogenous melatonin in pathogenesis of acute gastric
bleeding erosions induced by stress. J Physiol Pharmacol.
2007; 6:53-64.
7. Bülbüller N, Do¤ru O, Umaç H, Gürsu F, Akpolat N. The effects
of melatonin and pentoxiphylline on L-arginine induced acute
pancreatitis. Ulus Travma Acil Cerrahi Derg. 2005; 11:108-114.
8. Cantürk NZ, Sayek ‹. Cerrahi Araflt›rma. Ankara 2005:389-401.
9. Dabrowski A, Konturek SJ, Konturek JW, Gabryelewicz A. Role
of oxidative stress in the pathogenesis of caerulein-induced
acute pancreatitis. Eur J Pharmacol. 1999; 377:1-11.
10. Chen HM, Chen JC, Ng CJ, Chiu DF, Chen MF. Melatonin reduces
pancreatic prostaglandins production and protects against
caerulein-induced pancreatitis in rats. J Pineal Res. 2006; 40:34-
39.
11. Demircan O, Sönmez H, Ersöz C, Atay A. Koledok ve pankreatik
kanal ligasyonuyla akut pankreatit. Ulus Travma Acil Cerrahi
Derg. 1997; 3:175-180
12. Estebauer H, Cheeseen KH. In Packer C, Glazer AN. Oxygen radicals
in biological systems, methods in enzymology. California:
Academic Pres 1990: 407-421.
13. Eflrefo¤lu M, Gül M, Atefl B, Batçio¤lu K, Selimo¤lu MA. Antioxidative
effect of melatonin, ascorbic acid and N-acetylcysteine
on caerulein-induced pancreatitis and associated liver injury
in rats. World J Gastroenterol. 2006; 12:259-264.
14. Frossard JL, Steer ML, Pastor CM. Acute pancreatitis. Lancet.
2008; 371:143-152.
15. Fujimoto K, Hosotani R, Wada M, Lee J, Koshiba T, Miyamoto
Y, Doi R, Imamura M. Ischemia-reperfusion injury on the pancreas
in rats: identification of acinar cell apoptosis. J Surg Res.
1997; 71:127-136.
16. Hoffman RA & Reiter RJ. Rapid pinealectomy in hamsters and
other small rodents. Anatomical Record. 1965; 24:83-89.
17. Jaworek J, Konturek SJ, Tomaszewska R, Leja-Szpak A, Bonior
J, Nawrot K, Palonek M, Stachura J, Pawlik WW. The circadian
rhythm of melatonin modulates the severity of caerulein-induced
pancreatitis in the rat. J Pineal Res. 2004; 37:161-170.
18. Jaworek J, Brzozowski T, Konturek SJ. Melatonin as an organoprotector
in the stomach and the pancreas. J Pineal Res. 2005;
38:73-83.
19. Jaworek J, Nawrot-Porabka K, Leja-Szpak A, Bonior J,
Szklarczyk J, Kot M, Konturek SJ, Pawlik WW. Melatonin as
modulator of pancreatic enzyme secretion and pancreatoprotector.
J Physiol Pharmacol. 2007; 6:65-80.
20. Jaworek J, Nawrot K, Konturek SJ, Leja-Szpak A, Thor P, Pawlik
WW. Melatonin and its precursor, L-tryptophan: influence on
pancreatic amylase secretion in vivo and in vitro. J Pineal Res.
2004; 36:155-164.
21. Kaiser AM, Saluja AK, Sengupta A, Saluja M, Steer ML. Relationship
between severity, necrosis, and apoptosis in five models
of experimental acute pancreatitis. Am J Physiol. 1995;
269:1295-1304.
22. Konturek SJ, Konturek PC, Brzozowski T, Bubenik GA. Role of
melatonin in upper gastrointestinal tract. J Physiol Pharmacol.
2007; 6:23-52
23. Konturek SJ, Konturek PC, Brzozowska I, Pawlik M, Sliwowski
Z, Cze_nikiewicz-Guzik M, Kwiecie_ S, Brzozowski T, Bubenik
GA, Pawlik WW. Localization and biological activities of
melatonin in intact and diseased gastrointestinal tract (GIT). J
Physiol Pharmacol. 2007; 58:381-405.
24. Lerch MM, Saluja AK, Dawra R, Ramaraò P, Saluja M, Steer
ML. Acute necrotizing pancreatitis in the opossum: earliest
morphological changes involve acinar cells. Gastroenterology.
1992; 103:205-213.
25. Lowry O, Rosenbraugh N, Farr L, Randall RJ. Protein measurement
with the Folin-phenol reagent. J Biol Chem 1951; 193:265-
275.
26. Muñoz-Casares FC, Padillo FJ, Briceño J, Collado JA, Muñoz-
Castañeda JR, Ortega R, Cruz A, Túnez I, Montilla P, Pera C,
Muntané J. Melatonin reduces apoptosis and necrosis induced
by ischemia/reperfusion injury of the pancreas. J Pineal Res.
2006; 40:195-203.
27. Palao¤lu OS, Beflkonakl› E. Pineal gland and aging. Turkish Journal
of Geriatrics. 1998; 1:13-18.
28. Park BK, Chung JB, Lee JH, Suh JH, Park SW, Song SY, Kim
H, Kim KH, Kang JK. Role of oxygen free radicals in patients
with acute pancreatitis. World J Gastroenterol. 2003; 9:2266-
2269.
29. Sun E, XU Hi Liu Q et al. The mechanism for the effect of selenium
supplementation on immunity. Biol Trace Elem Res 1995;
48:231-238.
30. Walker NI. Ultrastructure of the rat pancreas after experimental
duct ligation. The role of apoptosis and intraepithelial macrophages
in acinar cell deletion. Am J Pathol. 1987; 126:439-451.
31. Warzecha Z, Dembinski A, Ceranowicz P, Dembi_ski M, Kownacki
P, Konturek SJ, Tomaszewska R, Stachura J, H_adki W,
Pawlik WW. Immunohistochemical expression of FGF-2,
PDGF_A, VEGF and TGF-_ RII in the pancreas in the course of
ischemia/reperfusion-induced acute pancreatitis. J. Physiol Pharmacol
2004; 55:791-810.
32. Weber H, Merkord J, Jonas L, Wagner A, Schröder H, Käding
U, Werner A, Dummler W. Oxygen radical generation and acute
pancreatitis: effects of dibutyltin dichloride/ethanol and ethanol
on rat pancreas. Pancreas. 1995; 11:382-388.

Thank you for copying data from http://www.arastirmax.com