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Sıçanlarda melatonin hormonunun tiroid folliküler hücreleri üzerine etkisi: AgNOR boyama ve elektron mikroskobik çalışma

Effects of melatonin hormone on the thyroid follicular cells in rats: AgNOR staining and electron microscopic study

Journal Name:

  • Selçuk Tıp Dergisi

Publication Year:

  • 2003

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Author NameUniversity of Author
Abstract (2. Language): 
This study was undertaken to examine effects of pinealectomy and pinealectomy followed by melatonin adminis-tration on thyroid gland. For this purpose 18 male VVistar rats were used. Animals were divided into three groups. Group I and II were designated as sham-pinealectomized (controi) and pinealectomized, respectively. The rats in Group III were pinealectomized and daily injected with melatonin for 1 month. At the end of the experiment, ali animals were killed by vascular perfusion. The thyroid glands of rats were removed, then some of the thyroid tissue specimens were stained with AgNOR techm'que and examined by light microscopy. AgNOR numbers in nuclei of thyroid follicular cells were counted. Some of the tissue specimens were examined by electron microscopy. İn our study, some findings proving the increase of mitoz activation along with the increase in the number of AgNOR granules in celi nuclei of thyroid epithel were observed after pinealectomy. Furthermore, lizozomes and secretory granules İn the cytoplasm of thyroid follicular cells were İncreased. İn the melatonin injected group, the number of AgNOR granules in celi nuclei were decreased. Additionally, ultrastructural changes, which indicate the increase of celi activation and is visible in thyroid epithel cells after pinealectomy were disappeared following injection of melatonin. İn conclusion, it was observed that there has been an increase in thyroid epithel celi proliferation and in celi activation after pinealectomy and this increase has been reversed by melatonin injection.
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Abstract (Original Language): 
Bu çalışma, pinealektominin ve pinealektomi sonrası uygulanan melatoninin tiroid bezi üzerine etkisinin araştırılması amacıyla yapıldı. Bu amaçla 18 adet VVistar-Albino cinsi erkek sıçan kullanıldı. Hayvanlar üç gruba ayrıldı. Grup I ve Grup II sırasıyla kontrol (Sham-pinealektomi) ve pinealektomili sıçanlar olarak düzenlendi. Grup IH'dekİ sıçanlara da pinealektomi sonrası günlük olarak ve bir ay süresince melatonin enjeksiyonu yapıldı. Deney süresi sonunda hayvanlar vaskülerperfüzyonla öldürüldü. Daha sonra sıçanlardan alınan tiroid doku örneklerinden bir kısmı AgNOR tekniği ile boyandı ve ışık mikroskopta incelenerek tiroid hücre çekirdeklerindeki AgNOR sayıları belirlendi. Tiroid doku örneklerinin bir kısmında da elektron mikroskobik inceleme yapıldı. Çalışmamızda, pinealektomi sonrası tiroid epitel hücre çekirdeklerindeki AgNOR taneciklerinin sayısında artışla birlikte mitoz aktivasyon artışını gösteren bulgular gözlendi. Ayrıca hücre sitoplazmalarındaki lizozom ve salgı granüllerinde artış görüldü. Melatonin enjekte edilen grupta ise, hücre çekirdeklerindeki AgNOR taneciklerinin sayısında düşüş olduğu belirlendi. Bununla birlikte, pinealektomi sonrası tiroid epitel hücrelerinde gözlenen ve hücre aktivasyon artışını gösteren ultrastruktürel değişikliklerin melatonin uygulaması ile kaybolduğu tespit edildi. Sonuç olarak, pinealektomi sonrası tiroid epitel hücre proliferasyonunda ve hücre aktivasyonunda artış meydana geldiği, melatonin enjeksiyonu İle de bu artışın gerilediği görüldü.
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