Preparation And Characteristics Of Magnetic Nanoparticles With Chitosan For Food Bacterial Detection

A world wide foodborne illness due to viruses remains a major concern, despite improving hygienic conditions. Magnetic nanoparticles have attracted recent researchers for their intensive applications as biomaterials and magnetic storage materials. The goal of this study is to synthesize Mg- NP's by using ultrasonic-assisted chemical coprecipitation method under different conditions. The Synthesized Mg- NP's was coated with chitosan for capturing the bacterial concentration from contaminated food sample. Chitosan from amine group specifically binds with bacteria on their surface of most food pathogenic bacteria (Staphylococcus aureus). The binding characteristic of chitosan coated Mg- NP's with bacterial cell wall was obtained by using UV-Visible spectroscopy and Transmission Electron Microscopy. UV-Visible Spectroscopy gives the reading of absorbance when the binding of receptor proteins occured with the sample. By using TEM, the Mg NP's shape and size was investigated. The product consisted of ferrous, ferrite (Fe3O4) nanosized cubic particles with a high level of crystallinity. This kind of Chitosan-magnetite nanocomposite has the potential to be used in biosensors to identify and to detect foodborne pathogens.