Buradasınız

Anasayfa » Content

QUALITATIVE DETERMINATION OF BIOLOGICALLY ACTIVE CONSTITUENTS IN MEDICINAL PLANT CRUDE EXTRACTS BY THIN LAYER CHROMATOGRAPHY

Journal Name:

  • Advance Research in Pharmaceuticals and Biologicals

Publication Year:

  • 2013

Key Words:

Author Name
Abstract (2. Language): 
TLC is an analytical technique used for the qualitative and quantitative evaluation of Plant extracts. Qualitative determination by thin-layer chromatography of the biologically active and/or characteristic constituents of dried hydroethanolic or ethanolic extracts of the following three medicinal plants is reported: Emblica officinalis Gaertn., Withania somnifera L., Ocimum sanctum L. The chromatographic separation was carried out on silica gel 60 F254 TLC plates. Toluene : Ethyl acetate : Acetic acid : Formic acid (20:45:20:5, v/v/v/v), Hexane : Isopropanol (8:2, v/v), Toluene : Ethyl acetate (9.9:0.1 v/v) as the mobile phase. Detection was carried out with keeping in Iodine chamber, under UV light or Dragendorff's reagent and Vanillin Sulphuric acid. The method is rapid, simple, and suitable for routine qualitycontrol analysis of plant extracts.
Bookmark/Search this post with
FULL TEXT (PDF): 
PDF icon arastirmax-qualitative-determination-biologically-active-constituents-medicinal-plant-crude-extracts-thin-layer-chromatography.pdf
  • 4
540
542
  • English

REFERENCES

References: 

1. G. R. Chatwal, S. K. Anand. Instrumental methods of
chemical analysis, Himalaya publishing house, 5th
edition, 2.599-2.616.
2. D. A. Skoog, F. J. Holler and T. A. Nieman. Principles
of instrumental analysis, Saunders college
publishing, 5th edition, 761-766.
Parwez Alam et al., ARPB, 2013; Vol 3 (IV) ISSN 2250-0774
(RESEARCH ARTICLE)
542 | P a g e w w w . a r p b . i n f o
3. B. K. Sharma. Instrumental methods of chemical
analysis, Goel publishing house, Meerut, 6th edition.
4. A.V. Kasture, K. R. Mahadik, S. G. Wadodkar, H. N.
More. A textbook of pharmaceutical analysis,
Instrumental methods, Nirali Prakashan, vol.2, 9th
edition, 18-30.
5. A. H. Beckett, J. B. Stenlake. Practical pharmaceutical
chemistry, CBS publishers, 4th edition, 115-128.
6. P. Bonvallet, J. Amburgey-Peters. Experiment 1:
SPARTAN’02 exercise on lewis structures, polarity,
and thin layer chromatography, Chemistry 211, Fall
2006.
7. M. Ali and V. Agrawal. Thin-layer chromatography
of aromatic amines, Separation Science and
Technology, 37(2002), 363 - 377.
8. A. M. Botvin'eva. Application of thin-layer
chromatography for analysis of heavy petroleum
products, Plenum Publishing Corporation, 1985,
462-464.
9. A. A. Siddiqui, M. Ali. Practical Pharmaceutical
chemistry, 1997, Ist ed. CBS publishers and
distributors, New Delhi, pp. 126-131.
10. Indian Pharmacopoeia Ashwagandha, In: I.P 1985,
Ministry of Health and Family
welfare, Government of India, Controller of Publicat
ions, Delhi, appendix 3.3:10:6.
11. J. B. Harborne. Phytochemical Methods: A Guide to
Modern Technique of Plant Analysis, Chapman and
Hall, London, 1984.
12. C. Cimpoiu, L. Jantschi, T. Hodisan. A new method
for mobile phase optimization in high-performance
thin-layer chromatography (HPTLC), J Planar
Chromatogra 11: 191-94 (1998).
13. P. D. Sethi, D. Charegaonkar. Introduction In:
Identification of Drugs in Pharamaceutical
Formulations by Thin Layer Chromatography, 2nd
ed. New Delhi, CBS publishers and Distributors,
2008, pp. 1-52.

Thank you for copying data from http://www.arastirmax.com