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ENANTİYOMERİK OLARAK SAF SEKONDER ALKOLLERİN ÜRETİMİNDE BİTKİ BİYOKATALİZÖRLERİ KULLANIMI

USE OF THE PLANT AS BIOCATALYSTS FOR PRODUCING ENANTİOMERİCALLY PURE SECONDER ALCOHOL

Journal Name:

  • Gazi Üniversitesi Mühendislik-Mimarlık Fakültesi Dergisi

Publication Year:

  • 2015

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
In this study, enantiomerically pure (S)-1-Phenylethanol was produced with bioreduction of acetophenone that the precursor of many pharmacological product. Derivatives of (S)-1-Phenylethanol (secondary alcohol) is used as the active ingredient of drug of anti alzheimer. Production of (S)-1-Phenylethanol was conducted with the asymmetric reduction reaction of acetophenone that biocatalyst for there action is enzyme of alcohol dehydrogenase (ADH). In this study, a variety of fruits and vegetables were used as source of enzyme (ADH) for reduction of the carbonyl group and ADH enzyme activities were determined of different plants (carrots, potatoes, grapes, radishes) for selection of suitable plant which it has the highest intra cellular enzyme activity. At the end of this election carrot has been considered as a biocatalyst which has highest ADH activity. The effects of size of biyocatalyst, source of enzyme, substrate concentration, temperature, reaction time, pH of there action and the amount of biocatalyst have been investigated on the production of (S)-1-Phenylethanol. It was found that ADH enzyme in fresh carrots catalysed the bioselective asymmetric reduction reactions at high conversion and high enantioselectively (>%99 ee and 92% c), under the conditions of 2mM substrate concentration, 200 g/L biocatalyst concentration, 1,52 mm biyocatalyst size, reaction temperature 33 °C, pH=7 and 150 rpm agitation speed.
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Abstract (Original Language): 
Bu çalışmada asetofenonun biyoindirgenmesiyle, birçok farmokolojik ürünün öncü maddesi olan enantiyomerik saflıkta (S)-1-fenil etanol üretilmiştir. (S)-1-fenil etanol türevleri antialzaymır ilaç etken maddesi olarak da kullanım alanına sahiptir. (S)-1-fenil etanolün üretimi, asetofenonun alkoldehidrojenaz (ADH) enzimi biyokatalizli asimetrik indirgenme tepkimesiyle gerçekleştirilmiştir. Çalışmanın ilk aşamasında, karbonil grubunu indirgeyebilmesi için ADH enzim kaynağı olarak çeşitli meyve ve sebzeler kullanılmıştır. En yüksek aktiviteye sahip olan uygun bitkinin seçimi için, farklı bitkilerin (havuç, patates, üzüm, turp) ADH enzim aktiviteleri tayin edilmiştir. Biyokatalizör olarak, en yüksek ADH aktivitesine sahip havuç seçilmiştir. Çalışmanın ikinci aşamasında, enantiyomerik saflıkta (S)-1-fenil etanol üretimine biyokatalizör boyutu, enzim kaynağı, substrat derişimive sıcaklık, tepkime süresi, tepkime pH’ıve hücre derişiminin etkileri araştırılmıştır. Enantiyoseçimli asimetrik biyoindirgenme tepkimesinde biyokatalizör olarak kullanılan taze havuç içindeki ADH enzimi tepkimeyi 2mM substrat derişimi, 200 g/L biyokatalizör derişimi, 1,52 mm biyokatalizör boyutu, 33 oC tepkime sıcaklığı, pH=7 ve 150 rpm karıştırma hızı koşullarında, yüksek enantiyoseçimlilikte ve yüksek dönüşümde katalizlemiştir (>%99 ee ve %92 c).
FULL TEXT (PDF): 
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