Buradasınız

Anasayfa » Content

Kuluçka Döneminde Hubbert Irkı Broylerlerde Sternum ve Costa’ların Kemikleşme ve Gelişiminin Belirlenmesi

OSSIFICATION AND GROWTH OF THE RIBS AND STERNUM IN PREHATCHING PERIOD OF THE HUBBERT STRAIN BROILER

Journal Name:

  • İstanbul Üniversitesi Veteriner Fakültesi Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Recent researches in experimental embryology of avian species have particularly investigated skeletal mutations, development of cultured embryos under artificial conditions, and teratogenic consequences of new drugs. Data on the normal skeletogenous stages are alsoessential parameters to interpret and elucidate the data acquired on these studies. Accordingly, a number of 10 embryos were prepared every 24 h from 10 to 20 days of incubation, to report the ossification and growth of the ribs and sternum of the Hubbert strain broiler (JV). The embryos fixed in 10% formaldehyde then 95% ethanol solutions were cleared and the skeletons of the embryos were stained in totowith Alcian blue&alizarin red for cartilage and ossified components, respectively. Observation of the skeletons photography were performed under a stereoscopic microscopy, paying particular attention to the timing ofchondrification and ossification of the bones. The first occurrence of the ossification was observed in the ribs on the 11 th day of incubation. Those in the sternum were seen on the 15 th day. We observed in this study that the uncinate processes of the ribs showed very early chondrification and ossification. The ossifiedcomponents continued to develop until the 20 th day of incubation when the hatching took place.
Bookmark/Search this post with
Abstract (Original Language): 
Son yıllarda kanatlılar üzerinde yapılan deneysel embriyolojik çalışmalar özellikle iskelet mutasyonları, yapay ortamlarda embriyo kültürlerinin gelişimi ve yeni ilaçların teratolojik etkilerini içermektedir. Normal iskelet gelişimi verileri, bu çalışmaların yorumlanmasıve doğrulanmasıiçin vazgeçilmez parametrelerdir. Araştırmada, inkubasyonun 10. gününden 20. güne kadar hergün 10 adet Hubbert (JV) ırkıembriyosu kuluçka makinesinden çıkartılarak sternum ve kostaların kemikleşmesi ve gelişimi gözlemlendi. %10’luk formaldehit solusyonunda ve % 95’lik ethanolde tespit edilen embriyolar temizlenerek, kıkırdak ve kemik dokuların ayrırenklerde tespiti için alcian blue ve alizarin red ile boyandı. İskelet sisteminin gözlenmesi ve fotoğraf çekimleri, özellikle kıkırdaklaşma ve kemikleşme zamanlarıdikkate alınarak, stereoskopik mikroskopla yapıldı. Kostalarda ilk kemikleşme merkezi inkubasyonun 11. gününde saptandı. Sternumun kemikleşmesinin ise 15. günde başladığıgözlendi. Çalışmada, kostalarda bulunan Proccessus uncinatus’ların çok erken kıkırdaklaşma ve kemikleşme sürecine başladığıtespit edildi. Kemikleşmişkısımların gelişiminin inkubasyonun 20. gününe kadar devam ettiği saptandı.
FULL TEXT (PDF): 
PDF icon arastirmax-kulucka-doneminde-hubbert-irki-broylerlerde-sternum-costalarin-kemiklesme-gelisiminin-belirlenmesi.pdf
  • 1
1-8
  • English

REFERENCES

References: 

1. Blom, J. and Lilja, C.: A comparative study of embryonic development of some bird
species with different patterns of postnatal growth. Zool.2005; 108: 81-95.
2. Blom, J. and Lilja, C.: A comparative study of growth, skeletal development and eggshell
composition in some species of birds. J. Zool. 2004. (London). 262: 361-369.
3. Etches, R.J., Carsience, R.S., Clark, M.E., Fraser, R.A., Toner, A., Verrinder
Gibbins,A. M.:Chimeric chickens and their use in manipulation of the chicken genome.
Poult. Sci, 1993; 72: 882-889.
4. Hamburger, H. and Hamilton H.L.: A serious of normal stages in the development of the
chick embryo. J. Morphol, 1951; 88: 49-92.
5. Hamilton, H.L.: Lillie’s development of the chick: An introduction to embryology.Holt,
Rinchart, Winston, New York. 1952.
6. Hashizume, R., Noda, A., Itoh, M., Yamamoto, Y., Masui, S., Oka, M.:A method for
detecting malformations in chicken embryos. Jap. Poult. Sci,. 1993; 30: 298-305.
7. Hashizume, R., Noda, A., Itoh, M.: Studies on teratological testing using chicken embryos:
effects of solvents, injection sites and the age of the embryo. Exp. Anim, 1992; 41: 349-356.
8. Jollie, M. T.: The head skeleton of the chicken and remarks on the anatomy of this region in
other birds. J. Morphol, 1957; 100: 389-436.
9. Lilja, C., Blom, J., Marks, H.L.: A comparative study of embryonic development of
Japanese quail selected for different patterns of postnatal growth. Zool, 2001; 104: 115-122.
10. Naito, M., Nirasawa, K., Oishi, T.: Development in culture of the chick embryo from
fertilized ovum to hatching. J. Exp. Zool, 1990; 254: 322-326.
11. Nakane, Y. and Tsudzuki, M.: Development of the skeleton in Japanese quail embryos.
Dev. Grow. Differ., 1999; 41: 523-534.
12.Nakane, Y. and Tsudzuki, M.: Morphological and genetic studies for a new morphometric
mutant of Japanese quail. Proceedings, 6
th
Asian Pacific Poultry Congress, 1998; 242-243.
13. Julian J.B.: Nomina Anatomica Avium, Handbook of Avian Anatomy Cambridge,
Massachusetts. Published by The Nuttall Ornithological Club. No: 23, 1993.
Ş.Hakan ATALGIN-İbrahim KÜRTÜL-Sami ÖZCAN-Emine Ü. BOZKURT 8
14. Ono, T., Matsumoto, T., Arisawa, Y.:Production of donor-derived offspring by transfer of
primordial germ cells in Japanese quail. Exp. Anim., 1998; 47: 215-219.
15. Ono, T., Murakami, T., Mochii, M.:A complete culture system for avian transgenesis,
supporting quail embryos from the single-cell stage to hatching. Dev. Biol, 1994; 161: 126-130.
16. Padgett, C.S. and Ivey, W.D.:The normal embryology of the coturnix quail. Anat. Rec,
1960; 137: 1-11.
17. Perry, M.M.: A complete culture system for the chick embryo. Nature, 1988; 331, 70-72.
18.Peters, P.W.J., Neubert, D., Merker, H. J., Kwasogroch, T. E.:Double staining of foetal
skeletons for cartilage and bone. Methods in prenatal toxicology. George Thieme, Stuttgart,
1977.
19.Ricklefs, R.E., Starck, J. M., Konarzewski, M.:Internal constraints on growth in birds.
Avian growth and development. Evolution within the altricial-precocial spectrum. pp. 266-287. In: Stack, J. M., Ricklefs, R. E. (eds.), Oxford University Press., Oxford. 1998.
20. Tsudzuki, M., Nakane, Y., Wada, A.:Hereditary multiple malformation in Japanese quail:
A possible powerful animal model for morphogenetic studies. J. Hered., 1998; 89: 24-31.
21. Zacchei, A.M.:Lo sviluppo embrionale della guaglia giapponese. Arch. It. Anat. Embriol.
1961; 66: 36-62

Thank you for copying data from http://www.arastirmax.com