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FarklıSığır Irklarına Ait Süt Örneklerinde B 12Vitamin Düzeyinin Elisa Tekniği İle Tespiti

DETECTION OF VITAMIN B 12IN DIFFERENT COW MILK USING ELISA TECHNIQUE

Journal Name:

  • İstanbul Üniversitesi Veteriner Fakültesi Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Vitamin B12 is a member of the vitamin B complex. It contains cobalt, and is also known as cobalamin. It is exclusively synthesised by bacteria and is found primarily in fish, meat, poultry, eggs, milk and milk products. Vitamin B12deficiency have an underlying stomach orintestinal disorder that limits the absorption of vitamin B12and might be caused to megaloblastic anemia. In this study cow milk samples were collected from in two different region in Turkey. In milk samples the amount of vitamin B12was detected by using ELISA. The vitamin B12concentrations of milk samples obtained from the cows of Holstein, Jersey,
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Abstract (Original Language): 
B12 vitamini, B kompleks vitaminlerin bir üyesidir. Bu vitamin kobalt içerdiğinden dolayı kobalamin olarak adlandırılmaktadır. B12 vitamini balık, et, tavuk, yumurta ve süt ürünlerinde bulunur ve bakteriler tarafından sentezlenir. B12 vitamini yetersizliğine bağlıolarak mide ve bağırsak bozukluklar, kol ve bacaklarda uyuşukluk ve megolablastik anemi şekillenir. Bu çalışmada farklısığır ırklarına ait süt örneklerinde B12vitamin düzeyleri araştırıldı. Bu amaçla Samsun ve Kars bölgesinden Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısısığır ırklarına ait süt örnekleri toplandı. Süt örneklerinde B 12 vitamini miktarı ELISA yöntemi ile tespit edildi. B12 vitamini Holştayn, Jersey, Zavot and Doğu Anadolu Kırmızısısığır ırklarına ait süt örneklerinde sırasıyla 0.98µg/L, 1.15µg/L, 2.93µg/L ve 1.56µg/L olarak bulundu. Yapılan istatistik çalışmasısonucunda vitamin B12miktarıZavot ırkına ait süt örneklerinde diğer ırklardan daha yüksek bulundu (p<0.05, ANOVA).Zavot and East Anatolian Red species were determined as 0.98µg/L, 1.15µg/L, 2.93µg/L and 1.56µg/L, respectively. The concentrations of vitamin B12in Zavot cows milk were higher than the other species.
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  • English

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