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DENEYSEL SICAK RENAL ÎSKEMÎ VE REPERFÜZYONDA LÎPÎD PEROKSÎDASYONU VE ALPHA-TOCOPHEROL TEDAVİSİNİN DEĞERLENDİRİLMESİ

THE EVALUATION OF LIPID PEROXIDATION AND ALPHA-TOCOPHEROL TREATMENT IN EXPERIMENTAL WARM RENAL ISCHEMIA AND REPERFUSION

Journal Name:

  • Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi

Publication Year:

  • 1996

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
We investigated effects of alpha-tocopherol on li-pid peroxidation and histological observations indu¬ced by oxygen free radicals generated during warm renal ischemia and reperfusion in 50 Guinea Pigs. Twenty-three Guinea Pigs were divided to five groups. Group 1 (n:10) was healthy controls. Group 2 (n:10) had warm renal ischemia for 30 minutes. Group 3 (n:10) was tretad intraperitoneal^ by alp-ha-tocopherol (100 mgper day) for three days before warm renal ischemia. Group 4 (n:10) had reperfusi-on for 60 minutes after warm renal ischemia for 30 minutes. Group 5 (n:10) was treated intraperitone-ally by alpha-tocopherol before warm renal ischemia and reperfusion. In Group 4, level of plasma malondialdehyde (me¬dian value: 9.52 nmollmL), an indicator of lipid pe-roxidation, was higher than those of Group 1, 2, 3 and 5 (6.67, 6.59, 6.56, 657 nmollmL, respectively; p<0.001 for all groups). Histological observations showed necrosis of pro¬ximal and distal convoluted tubules following warm renal ischemia. This necrosis area expanded, and glomerulus injury started following reperfusion. The treatment of alpha-tocopherol did not affect to histo-logical changes of renal ischemia, but it reduced re-perfusion injury after renal ischemia. Finally, it was shown that alpha-tocopherol redu¬ced both lipid peroxidation and histologic injury in reperfusion following warm renal ischemia.
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Abstract (Original Language): 
îskemi sonrası reperfüzyonla meydana gelen oksijen serbest radikalleri iskemik akut renal yetmezlik patogenezinde ve renal transplantasyon sonrası al-lograft fonksiyonunun gecikmesinde önemli rol oynar¬lar. Bu çalışmada 50 Guinea Pigde sıcak renal iske-mi ve reperfüzyon sırasında oluşan oksijen serbest radikalleriyle meydana gelen Lipid peroksidasyonu ve renal histolojik değişiklikler üzerinde alpha-tocophe-rol (E vitamini) tedavisinin etkilerini incelemeyi amaçladık. 50 Guinea Pig, 5 gruba bölündü. Grup 1 (n=10) sağlıktık kontrollerdi. Grup 2 (n=10)'ye 30 dk. sürey¬le sıcak renal iskemi uygulandı. Grup 3 (n-10)'e sı¬cak renal iskemi öncesi 3 gün süreyle intraperitoneal alpha-tocopherol (100 mg/gün) verildi. Grup 4 (n=10)'e 30 dk. sıcak renal iskemi sonrası 60 dk. re-perfüzyon yapıldı. Grup 5 (n-10)'e intraperitoneal alpha-tocopherol verildi ve sıcak renal iskemiyi taki¬ben reperfüzyon uygulandı. Grup 4'de lipid peroksidas)'onunun bir göstergesi olan plasma malondialdehit düzeyleri (median değer: 9.52 nmollmL) sırasıyla Grup 1,2,3 ve 5den daha yüksek bulundu (6.67, 6.59, 6.56, 6.57 nmollmL, p < 0.001 tüm gruplar için). Renal histolojik incelemede ise sıcak renal iskemi-yi takiben proksimal ve distal tubuluslarda nekroz gözlendi. Reperfüzyonu takiben bu nekroz sahası ge¬nişledi ve glomerulus hasarı başladı. Alpha-tocophe-rolle tedavi, renal iskeminin histolojik değişikliklerini etkilemedi, fakat renal iskemi sonrası reperfüzyon ha¬sarını azattı. Sonuç olarak, alpha-tocopherol'ün sıcak renal is-kemiyi takiben reperfüzyonda lipid peroksidasyonu ve renal histolojik hasarı azalttığı gösterildi.
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