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Tavşanda Deneysel Osteoartrit Modelinde İnsan Amniotik Sıvısının Eklem İçi Uygulamasının Kıkırdak Doku ve Sinovya Üzerindeki Etkileri

The Effects of Intra-Articular Injections of Human Amniotic Fluid in an Experimental Osteoarthritic Rabbit Model

Journal Name:

  • Uludağ Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2007

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
This experimental study aimed to investigate the effects of intra-articular injection of human amniotic fluid (HAF) on articular cartilage and sinovial tissue in a developed osteo-arthritic rabbit knee model. The right knee anterior cruciate ligament was cut to develop osteoarthritis in 20 rabbits. After 4 weeks, 10 rabbits were given intra-articular injections of 0.5ml HAF once a week for 3 weeks (Group I). The other 10 rabbits as control group received no treatment (Group II). Both groups were sacrificed 12 weeks after the final injection. The Meachim morphologic grading for macroscopic evaluation, and modified Mankin evaluation for histopathological cartilage examination, were used. Histological evaluation of the synovial tissue according to Yoshimi histopathologic grading and the synovial lining cell layer thickness was measured. According to morphologic evaluation, 100% of group I had grade 1-2, while were %80 of group II had grade 3-4 (p<0.05). In the histopathological evaluation of the cartilage, the medial femoral condyles and medial tibial plateau in Group I were 6.6 (1- 12) and 7.0 (1-12) respectively, while in Group II they were 17.8 (7-30) and 18.9 (6-30), (p<0.05). Significant differences were observed in the synovial tissue histopathologic grading (p<0.05; Group I 6.3 (3-8), Group II 12.9 (6-17). Mean synovial lining cell layer thickness was 20 µm (10-30) in Group I and 29 µm in Group II (p<0.05). Since HAF, which is an inert fluid, rich in proteins, macromolecules and growth factors, has protective effects against cartilage and synovial tissue changes in an experimental rabbit model, it may be an alternative agent in cartilage protective therapy.
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Abstract (Original Language): 
Bu deneysel çalışmada tavşanlarda oluşturulan deneysel osteoartrit modelinde eklem içi uygulanan insan amniyotik sıvı’nın (İAS) eklem kıkırdağı ve sinoviyal dokuya etkisinin araştırılması amaçlanmıştır. Osteoartrit geliştirilmek için 20 tavşanın sağ dizinin ön çapraz bağı kesildi. Dört hafta sonra 10 tavşana birer hafta ara ile 3 kez eklem içi 0,5 ml İAS enjeksiyonu uygulandı. (Grup 1). Diğer 10 tavşanda kontrol grubu olarak kaydedildi ve herhangi bir tedavi uygulanmadı. (Grup 2). En son uygulanan eklem içi enjeksiyondan 12 hafta sonra her iki grupdaki tavşanlar sakrifiye edildi. Eklem kıkırdak dokusunun makroskopik değerlendirilmesi için Meachim morfolojik evreleme sistemi, histopatolojik değerlendirmesi için modifiye Mankin değerlendirme sistemi kullanılmıştır. Sinoviyal doku histolojik değerlendirmesi Yoshimi’ye göre yapılmış ve sinoviyal hücre tabakası kalınlığı da ölçülmüştür. Makroskopik incelemede Grup 1’in %100’ü evre 1–2 iken Grup-2’nin %80’i evre 3–4 olarak değerlendirildi (p<0,05). Kıkırdağın histopatolojik değerlendirmesinde Grup 1’de medial femoral kondil ve medial tibial plato sıra ile 6,6 (1–12) ve 7,0 (1–12), Grup 2 de ise 17,8 (7–30) ve 18,9 (6–30) olarak tespit edildi (p<0,05). Sinoviyal doku histopatolojik derecelendirmesinde belirgin istatistiksel farklılık tespit edildi (p<0,05; Grup–1 6,3 (3–8), Grup–2 12,9 (6– 17)). Ortalama sinoviyal hücre tabakası kalınlığı Grup 1’de 20 µm (10–30) Grup 2’de 29 µm olarak ölçüldü (p<0,05). Sonuç olarak proteinlerden, makromoleküllerden ve büyüme faktörlerinden zengin inert bir sıvı olan İAS’ın, tavşan deneysel osteoartrit modelinde kıkırdak ve sinoviyal dokudaki dejeneratif değişikliklerine karşı koruyucu etkisinin olması nedeniyle kıkırdak koruyucu tedavi ajanlarına bir alternatif olabileceği kanısına varıldı
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  • 3
121-125
  • Turkish

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