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ÇEŞİTLİ ZORUNLU ANAEROP BAKTERİLERİN DENTIN TUBULLERINE PENETRASVONLARININ SEM' DE (Scanning Electron Microscope) İNCELENMESİ

EVALUATION OF TUBULAR PENKTKA HON OF VARIOUS OBLIGATE ANAEROB MICROORGANISMS ( A SEM Study)

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 2000

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The obligate anaerob microorganisms which play an important role in the pathogenesis of the pulp diseases and periapical pathology can penetrate into the dentinal tubules before or during Ihe endodontic therapy and they can prevent themselves from the effects of endodontic files and irrigation solutions. The aim of this study was to evaluate the dentinal tltbuie penetration abilities of the obligate anaerûb microorganisms • K nucleatum and B. melaııinogenicus which were resistant to the endodontic therapy. 18 upper incisor teeth were used in this study. The cylindrical dentinal blocks which were prepared from these teeth were incubated for 21 days in the culture medium including these microorganisms. At ihe end: of this period Ihe samples were fixed in 1$ % ylutaraldehid solution. Bacterial colonics and their penetrations into the dentinal tubules were evaluated under SEM. As a result both microorganisms appeared colonisation on the dentin surface, but very few of Ihem penetrated into the dentinal tubules due to their morphologies and it was seen that they only would go to a shoix distance.
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Abstract (Original Language): 
Pulpu ve periapikal doku hastalıklarının pattı gene zinde önemli mi ojnayan /.orunlu anaerop mikroorganizmalar, tedavi öncesi ya da tedavi sırasında denlin tubüllerine yayılarak endodontik aletlerin vc İrrigasyon solüsyonlarının etkisinden korunurlar. Çalışmamızda endodontik tedaviye direne gösteren enfeksiyonlarda bulunan zorunlu anacrap mikroorganizmalardan F.nuclealum ve B. mdaninogenieus'un denlin tLibüllerine penelrasyon kabiliyetleri SEM'dc (Scanning electron microscope) incelendi. Bu amaçla 18 ndct üst keser dişten elde edilen sihndırik dentin blokları her iki mikroorganizmayı içeren be si ye derine konularak 21 günlük inkübasyona bırakıldı. Bu süre sonunda örnekler °k. 2-5' Ink glutaıaldchil solüsyonunda fikse edilerek SKM'de dentin yüzeyinde bakteri kolonizasyonu ve dentin tilbiilierine penetrasyonları yönünden incelendi. Sonuç olarak her iki mikroorganizmanın da denlin yüzeyinde yoğun kolonizasyon oluşturdukları ancak az sayıda bakterinin dentin tübüllcrinc girebildiği vc kısa meşalede ilerleyebildiği tesbit edildi
FULL TEXT (PDF): 
PDF icon arastirmax-cesitli-zorunlu-anaerop-bakterilerin-dentin-tubullerine-penetrasvonlarinin-sem-de-scanning-electron-microscope-incelenmesi.pdf
  • 1
26-31
  • Turkish

REFERENCES

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