Journal Name:
- Advance Research in Pharmaceuticals and Biologicals
| Author Name |
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Abstract (2. Language):
A rapid and sensitive reverse phase high performance liquid chromatography (RP-HPLC) method has been developed
for estimation of Granisetron hydrochloride in bulk and pharmaceutical dosage forms. Granisetron hydrochloride was
chromatographed on a reverse phase kromasil C18 column (250 x 4.6mm; 5μm) in a mobile phase consisting of 0.05 M
potassium dihydrogen phosphate buffer (pH 3.0 adjusted with orthophosphoric acid) and acetonitrile in the ratio of
70:30. The mobile phase was pumped at a flow rate of 1.0 mL/min with detection at 301 nm. The retention time for
Granisetron hydrochloride was 4.05min.The detector response was linear in the concentration of 1μg/mL-18μg/mL
with correlation coefficient of 0.9996. The percentage recovery of granisetron hydrochloride at target concentration was
found to be 94.9 %. The limit of detection and limit of quantification was found to be 0.50 μg/ml and 0.25 μg/ml
respectively. Method validation parameters were found to be within the specified limits. The proposed method was
found to be simple, fast, accurate, precise and reproducible and could be used for routine quality control analysis of
granisetron hydrochloride in bulk and Granisetron hydrochloride mouth dissolving film.
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