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ACTINOBACILLLUS ACTINOMYCETEMCOMIT ANS A KARŞI FARKLI ANTÎBİOTİKLERİN İN VİTRO AKTİVİTELERİ

IN VITRO ACTIVITY OF DIFFERENT ANTIBIOTICS AGAINST ACTINOBACIIXUS ACTINOMYC ETEMC OM1TA NS

Journal Name:

  • Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 1998

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Actinobacıllus actinomycetemcomitans is a gram negative, facultative anaerob coccobacillus that plays an important role in the etiology of especially localized juvenile periodontitis- The aim of this study was to evaluate this microorganism's susceptibility to different antibiotics, 10 localized juvenile periodontitis patients were included in the study- Subgingival microbiologic samples were taken from the deepest pocket of first molars İn each quadrant with paper points. Samples were than immediately cultured on Tryplic Soy-Scrum-Bacitracin-Vancomycin agar plates. They were incubated at 37°C for 3 days. At the end of this incubation period microorganisms were evaluated according to their colony morphologies and then, they were gram stained and tested for oxidase and catalase activity. All localized juvenile periodontitis patients were culture positive for subgingival Actinobacillus actinomycetemcomitans- Antibioti c susceptibility tests of 10 Actmobacillus actinomycetemcomitans isolates were performed by using standard antibiotic discs on Columbia agar. Among the six antibiotics, all of the Actinobacillus actinomycetemcomitans isolates were susceptible to tetracycline, ofloxacme and azithromycin.
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Abstract (Original Language): 
Actinobicallus actinomycetemcomitans özellikl e lokalize jiivenil periodontitisde etiolojik ajan oiarak tanımlanan gram negatif, fakiiltatif anaerob bir kokobasildir ÇalışmaniTi amacını bu mikroorganizmanın farklı antibiotiklere olan duyarlılığının incelenmesi oluşturmuştur. Çalışma, lokaüze jüvenil periodontitis tanısı konan 10 hasta üzerinde gerçekleştirilmiştir- Subgingival mikrobiyolojik örnekler steril paper pointier ile her quadrantdaki en derili cebe sahip birinci molar dişlerden alınmıştır. Örnekler alındıktan hemen sonra Tryptie Soy-Senım-Bacilracin-Vancanıyein besi yerlerine ekilmiştir, Besi yerleri anaerobik jara yerleştirilerek 37°C'da 3 gün süreyle inkübe edilmiştir. İnkübasyon periodunu takiben izlenen mikroorganizmalar koloni morfolojileri, gram boyası, oksidaz ve katalaz reaksiyonu yününden değerlendirilmiş ve lıastalarm tümünde Actinobacillus actinomycetemcomitans mevcudiyeti saplanmıştır. İzole edilen 10 Actinobacillus actinomycetemcomitans suşunda da farklı antıbiürikleri içeren standart disklerle Columbia ağarda antibiotik duyarlılık testi yapılmıştır. Test edilen6 antibiotikden 10 Actinobacıllus actinomycetemcomitans susuna da duyarlı olanların tetrasiklin, ofloksasin ve a/.itromisin olduğu gözlenmiştir.
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  • Turkish

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