You are here

Ratlarda sol kolon greftinde eritropoetinin iskemi üzerine koruyucu etkileri

The protective effects of erythropoietin on ischemia in the left colonic graft in rats

Journal Name:

Publication Year:

Abstract (2. Language): 
Segments from the stomach, jejunum, ileum and colon may be used for esophageal replacement when irreversible injury of the esophagus prevents passage. Of these techniques colon interposition is the most preferred method. Reperfusion damage develops at a certain rate in colon grafts used for esophageal replacement as with all other surgical methods where grafts are used. It is known that erythropoietin hormone may have a tissue-protective effect in ischemia/ reperfusion. Ischemic changes developing in mucosal cells in the graft ends after creating a left colon graft in rats and the effect of intraperitoneal erythropoietin administration on these changes were investigated in the present study. A total of 56 subjects were randomly divided into four main groups of Sham (n=14), Sham+erythropoietin (n=14), colon graft (n=14) and colon graft+erythropoietin (n=14), and each group was divided into 2 subgroups. Histopathological examination was performed to the first subgroup one day and to the second subgroup 7 days after reoperation. There was significantly much more mucosal damage in the colon graft group in comparison to the postoperative day 1 and 7 proximal ends between the colon graft and colon graft+erythropoietin groups. Results of the present study showed that intraperitoneal erythropoietin administration significantly decreased mucosal damage in rats in which a colon graft was prepared.
Abstract (Original Language): 
Ösefagusun geriye dönüşsüz bir şekilde hasar görerek pasajı engellediği durumlarda, ösefagus replasmanı amacıyla mide, jejunum, ileum ve kolon segmentleri kullanılabilmektedir. Bu tekniklerden kolon interpozisyonu en sık tercih edilen yöntemdir. Greftlerin kullanıldığı tüm diğer cerrahi yöntemler gibi ösefagus replasmanı amaçlı kullanılan kolon greftlerinde de reperfüzyon hasarı belirli oranda gelişmektedir. Eritropoetin hormonunun iskemi/reperfüzyonda dokuyu koruyucu bir etki gösterebileceği bilinmektedir. Bu çalışmada ratlarda, sol kolon grefti oluşturulduktan sonra greftin uç kısımlarında meydana gelen mukozal hücrelerdeki iskemik değişiklikler ile intraperitoneal eritropoetin uygulamasının bu değişiklikler üzerine olan etkileri araştırılmıştır. Toplam 56 denek rastgele Sham (n=14), Sham+eritropoetin (n=14), kolon grefti (n=14), kolon grefti+eritropoetin (n=14) olmak üzere dört ana gruba, her grup da kendi içinde 2 alt gruba ayrıldı. Birinci alt gruba 1 gün sonra, 2. alt gruba ise 7 gün sonra reoperasyon sonrası histopatolojik inceleme yapıldı. Kolon grefti ve kolon grefti+eritropoetin grupları arasında postoperatif 1. gün ve 7. gün proksimal uçlarının değerlendirilmesinde mukozal hasarın kolon grefti grubunda anlamlı olarak daha yüksek olduğu görüldü. Bu çalışma, preoperatif intraperitoneal eritropoetin uygulamasının, kolon grefti hazırlanmış ratlarda mukozal hasarlanmayı belirgin olarak azalttığını göstermektedir.
233-237

REFERENCES

References: 

Kaynaklar
1. Ngan SY, Wong J. Lenghts of different routes for
esophageal replacement. J Thorac Cardiovasc Surg
1986; 91: 790-792.
2. Tannuri U, Maksoud Filho JG, Maksoud JG.
Esophagoplasty in children: surgical technique with
emphasis on the double blood supply to the interposed
colon, and results. J Pediatr Surg 1994; 29: 1434-1438.
3. Jeffrey HP, Jeffrey WK, Michael K. Arterial anatomic
considerations in colon interposition for esophageal
replacement. Arch Surg 1995; 130: 858-862.
4. Bassiouny IE, Al-Ramadan SA, Al-Nady A. Long-term
functional results of transhiatal oesophagectomy and
colonic interposition for caustic oesophageal stricture.
Eur J Pediatr Surg 2002; 12: 243-247.
5. Tsao CK, Chen HC, Chuang CC, et al. Adequate venous
drainage: the most critical factor for a successful free
jejunal transfer. Ann Plast Surg 2004; 53: 229-234.
6. Henrich DE, Lewis RS, Logan TC, Shockley WW. The
influence of arterial insufficiency and venous congestion
on composite graft survival. Laryngoscope 1995; 105:
565-569.
7. Krantz SB. Erythropoietin. Blood 1991; 77: 419-434.
8. Cerami A. Beyond erythropoiesis: novel applications for
recombinant human erythropoietin. Semin Hematol
2001; 38: 33-39.
9. Ledbetter DJ, Juul SE. Erythropoietin and the incidence
of necrotizing enterocolitis in infants with very low
birth weight. J Pediatr Surg 2000; 35: 178-182.
10. Juul SE, Joyce AE, Zhao Y, et al. Why is erythropoietin
present in human milk? Studies of erythropoietin
receptors on enterocytes of human and rat neonates.
Pediatr Res 1999; 46: 263-268.
11. Felix WL, Kenny CS, Jose MP. Superior mesenteric
artery is more important than inferior mesenteric artery
in maintaining colonic mucosal perfusion and integrity
in rats. Dig Dis Sci 1992; 37: 1329-1335.
12. Peter J, Ido S, Jehuda I, Lael-Anson B. Delivery following
colon interposition. Chest 2003; 124: 2027-2028.Cilt 50 • Sayı 4 Eritropoetinin sol kolon greftindeki etkisi • 237
13. Murthy S, Hui-Qi Q, Sakai T. Ischemia/reperfusion injury
in the rat colon. Inflammation 1997; 21: 173-190.
14. Juul SE, Ledbetter DJ, Joyce AE. Erythropoietin acts as
a trophic factor in neonatal rat intestine. Gut 2001; 49:
182-189.
15. Fatouros MS, Vekinis G, Bourantas KL. Influence
of growth factors, erythropoietin and granulocyte
macrophage colony stimulating factor on mechanical
strength and healing of colonic anastomoses in rats.
Eur J Surg 1999; 165: 986-992.
16. Felix WL, Kenny CS, Yoshikazu Y. Regional differences
in mucosal hemodynamics in experimental colonic
injury in rats. Dig Dis Sci 1993; 38: 1220-1223.
17. Zimmerman JB, Grisham MB, Granger N. Role of
oxidants in ischemia/reperfusion-induced granulocyte
infiltration. Am J Physiol 1990; 21: 185-190.

Thank you for copying data from http://www.arastirmax.com