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Ratlarda lipid peroksidasyonunun indüklediği spinal kord hasarında melatoninin etkisi: malondialdehitin tanısal önemi

Effect of melatonin on spinal cord injury induced lipid peroxidation in rats: prognostic value of malondialdehyde

Journal Name:

  • Süleyman Demirel Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi

Publication Year:

  • 2011

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Aim: Melatonin is a potent antioxidant agent produced by the pineal gland. In this study we aimed to investigate the neuro-protective effect of melatonin on ischemic spinal cord in the rats. Method: Aortic clamp was used for the occlusion of the abdominal aorta for 30 minutes. Twenty four Winstar male rats were used for this study and rats were divided randomly into 4 groups: control group (sham group, n: 5), sham+melatonin treated group (receiving 10mg/kg melatonin intraperitoneally, n: 5), ischemia/reperfusion group (undergoing occlusion but receiving no pharmacologic intervention, n: 7), melatonin + ischemia/reperfusion group (undergoing occlusion and receiving 10mg/kg melatonin intraperitoneally 10 minutes after ischemia, n: 7). Spinal cord samples were taken for the malondialdehyde (MDA) analyses. Results: In the aort occlusion group, the MDA levels, indicating the extend of lipid peroxidation, were significantly higher than the other groups (p<0.05). Also in the aort occlusion + melatonin group the levels of MDA were significantly reduced compared to the aort occlusion group (p<0.05). Discussion: In this study, we showed that injection of melatonin reduces the effects of secondary injury and facilitate the recovery of the damaged spinal cord.
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Abstract (Original Language): 
Amaç: Melatonin, pineal bez tarafından üretilen güçlü bir antioksidan ajandır. Bu çalışmada biz, ratlarda spinal kord iskemisinde melatoninin nöroprotektif etkisini araştırmayı amaçladık. Yöntem: Abdominal aortun oklüzyonu için 30dk süre ile aortic klemp kullanıldı. Çalışmada 24 Winstar erkek rat kullanıldı ve ratlar rastlantısal olarak 4 gruba ayrıldı: kontrol grubu (sham grup, n:5), sham + melatonin verilen grup (10mg/kg melatonin intraperitoneal olarak verildi, n:5), iskemi/reperfüzyon grubu (oklüzyon yapılan ancak herhangi bir farmakolojik ajan verilmeyen grup, n:5), melatonin + iskemi/reperfüzyon grubu (oklüzyon yapılan ve iskemiden 10 dk sonra intraperitoneal olarak 10mg/kg melatonin verilen grup). Spinal kord örnekleri malondialdehit (MDA) analizi için alındı. Sonuçlar: Aort oklüzyonu yapılan grupta, lipid peroksidasyonunun olduğunu gösteren MDA düzeyleri diğer gruplardan belirgin olarak daha yüksekti (p<0.05). Aort oklüzyonu+melatonin verilen grupta da aort oklüzyonu yapılan grup ile karşılaştırıldığında MDA düzeylerinin belirgin olarak azaldığı görüldü (p<0.05). Tartışma: Bu çalışmada biz, melatonin enjeksiyonunun sekonder hasarın etkilerini azalttığını ve hasarlı spinal kordun iyileşmesini kolaylaştırdığını gösterdik.
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  • 2
65-69
  • English

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