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Cape'nin Süperoksit Süpürücü Kapasitesinin In Vitro incelenmesi

Investigation of In Vitro Superoxide Scavenging Capacity of Cape

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2002

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Keywords (Original Language):

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Abstract (2. Language): 
introduction: The antioxidant capacity of CAPE (caffeic acid phenethyl ester) has been investigated in recent years but, so far most of the studies were in vivo studies. in this study we investigated, the in vitro antioxidant capacity of CAPE to scavenge superoxide radicals. CAPE, an active component of propolis from honeybee hives, is known to have anti mitogenic, antioxidant, anticarcinogenic, antiinflammatory and immunomodulatory properties. Aim of the study: CAPE is an antioxidant molecule in vivo. The aim of this study is to investigate if CAPE under in vitro conditions has the same effect similar to in vivo. Methods: The method developed by McCord and Fridovich for the determination of SOD activity is modified by replacing SOD with CAPE. Results: We find that, CAPE has superoxide scavenging capacity in a dose dependent manner. The presence of 50 iimol/L CAPE caused 73.66 % percentage of inhibition in cyt c reduction.
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Abstract (Original Language): 
Giris:Son yillarda CAPE (Kafeik asi t fenil esteri)'nin antioksidan kapasitesi arastirilmistir, ancak su ana kadar yapilmis olan çalismalarin çogu in vivo çalismalardir. Biz bu çalismada CAPE'nin süperoksit radikallerine karsi antioksidan kapasitesini in vitro arastirdik. CAPE bal arisi kovani propolisinin aktif bir bileseni olup antimitojenik, antioksidan, antikarsinojen, antiinflamator ve immünomodülatör özellikleri bilinmektedir. Amaç: CAPE in vivo antioksidan bir moleküldür. Bu çalismada, CAPE'nin benzer kosullarda ancak in vitro ortamda ayni etkiyi gösterip göstermediginin arastirilmasi amaçlandi. Metod: McCord ve Fridovich'in SOD aktivitesi tayini için gelistirdikleri yöntem, SOD yerine CAPE kullanilarak modifiye edildi. Bulgular: CAPE'nin doza bagimli olarak artan bir süperoksit süpürücü kapasiteye sahip oldugunu saptadik. 50 ? mol/L CAPE varliginda cyt c redüksiyonu %73,66 inhibe oldu.
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REFERENCES

References: 

1. Pierrefiche G., Laborit H., Oxygen free radicals, melatonin and aging. Experimental Gerontology, 1995, 30:213-227.
2. Buechter D.D., Free radicals and oxygen toxicity. Pharm. Res. 1988, 5:253-260.
3. Halliwell B., Tell me about free radicals, doctor: A rewiev. J. Royal Soc.Med. 1989, 82:747-752.
4. Gutteridge J., Halliwell B., Oxygen toxicity, oxygen radicals, transition metals and diseases. Rewiev article. Biochem. J. 1984, 219:1-14
5. McCord J.M., Free radicals and myocardial ischemia:Overview and outlook. Free
Rad. Biol Med. 1988, 4:9-15.
6. Sies H., Oxidative Stress, Academic Press inc. London., 1985, 16-21
7. McCord J.M., Fridovich i., The biology and pathology of oxygen radicals. Ann. int.
Med. 1978, 89:122-127.
8. Hayakawa H., Hofer A., Thelander L., Metabolic fate of oxidized guanine ribonücleotides in mammalian cells. Biochemistry, 1999, 38:3610-36145
9. Aust S.D., Svingen B.A., Free radical in biological systems. Acad. Press. London,
1985, 1-28.
10. Guemouri L., Yues A., Herbert B., Biological variability of SOD, CAT and GPx in blood. Clin. Chem. 1991, 37:932-937.
11. Burdock G.A., Review of the biological properties and toxicity of bee propolis. Food Chem. Toxicol. 1998, 36:347-363.
12. Kujumgiev A., Tsuetkova i., Bankova V., Antibacterial, antifungal and antiviral activity of propolis of different geographical origin. J. Ethnopharmacol. 1999, 64:235-340.
13. Marcucci M.C., Ferreres F., Garcia-Viguera C., Phenolic compounds rfom Brazilian propolis with pharmacologiocal activities. . J. Ethnopharmacol. 2001, 74:105-122.
14. Matsushige K, Basnet P, Kadota S., Potent free radical scavenging activity of dicaffeoyl quinic acid derivatives from propolis. J Trad Med. 1996, 13:217-228.
15. Laranjinha J., Cadenes E., Redox cycles of CAPE, alpha-tocopherol and ascorbate: implications for protection of LDL against oxidation. iUBMB Life, 1999, 48(1):57-
65
16. Michaluart P., Masferrer J.L., Carothers A.M., Subbaramaiah K, inhibitory effects of CAPE on the activity and expression of cyclooxygenase 2 in human oral epithelial cells and in a rat model of inflammation. Cancer Res. 1999,59(10):2347-52.
17. Krol W, Czuba Z, Scheller S, Gabry J., Anti-oxidant property of ethanolic extract of propolis as evaluated by inhibiting the chemiluminescence oxidation
of luminol. Biochem Int. 1990, 21(4):593-597.
18. McCord J.M., Fridovich I., An enzymatic function for eritrocuprein. J. Biol.
Chem., 1969, 244:6049-6055.
19. Grunberger D., Banerjee R., Eisinger K., Oltz EM., Efros L., Preferential cytotoxicity on tumor cells by caffeic acid phenethyl ester isolated from
propolis., Experientia, 1998, 44:230-232.
20. Lawrence Kaplan, Clinical Chemistry, Mosby Press, NY , 1996, pp:501.
21. Nomura M., Kaji A., Wei-ya M., Dong Z., Suppression of cell transformation and induction of apoptosis by caffeic acid phenethyl ester Molecular Carcinogenesis, 2001, 31(2):83-89
22. Frenkel K., Wie H., Bhimani R., Ye J., inhibition of tumor promotor mediated processes in mouse skin and bovine lens by CAPE. Cancer Res. 1993, 53(6):1255-61.
23. Cengarle L., Carta A., Tilloca G., Antioxidant activity of a Sardinian Propolis.
Riv. Ital. Sostanze Grasse, 1998, 75:551-557.
24. Suzuki H, Takai M, Koide H., Anti tumor effect of the immunoactivity fraction obtained from Brazilian water soluble pro-polis, given in combination with
8
Investigation of In Vitro Superoxide Scavenging Capacity of Cape
anticancer drug in Ehrlich carcinoma bearing mice. Honeybee Science, 1996,
17:1-6.
25. Basnet P, Matsushige K, Hase K, Kadota S., Four di-O-caffeoyl quinic acid derivatives from propolis. Potent hepatoprotective activity in experimental liver injury models. Biol Pharm Bull. 1996, 19(11):1479-84
26. Basnet P, Matsushige K, Hase K., Potent antihepatotoxic activity of dicaffeoyl quinic acids from propolis. Biol Pharm Bull. 1996,19(4):655-7
27. Merino N, Gonzalez R, Gonzalez A, Remirez D., Histopathological evaluation on the effect of red propolis on liver damage induced by CCi4 in rats. Arch Med Res. 1996, 27:285-289.
28. Banskota AH, Tezuka Y, Adnyana iK, Midorikawa K., Cytotoxic, hepatoprotective and free radical scavenging effects of propolis from Brazil, Peru, the Netherlands and China. J Ethnopharmacol. 2000, 72(1-2):239-46.
29. Maria I. Nieva Moreno, Maria I. Isla, Antonio R., Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J
Ethnopharmacol. 2000, 71(2):109-114
30. Antolin I, Rodriquez C, Sainz RM, Mayo JC Uria H., Neurohormone melatonin prevents cell damage; effect on gene expression for antioxidant enzymes.
FASEB J, 1996, 10:882-890

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