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MODULATORY INFLUENCE OF ALOE VERA AGAINST RADIATION AND CADMIUM INDUCED BIOCHEMICAL CHANGES IN THE BRAIN OF SWISS ALBINO MICE

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2013

Key Words:

Author Name
Abstract (2. Language): 
Humans are exposed to ionizing radiations during diagnostic, therapeutic and industrial purposes. Apart from these human also get exposed to ionizing radiations during air and space travel, background radiation, nuclear accidents and use of electronic devices. Therefore it is essential to protect humans from ionizing radiation. Majority of plant and herbs have medicinal properties and they protect against the radiation induced damage by scavenging free radicals and increasing antioxidant status. Aloe vera has a long history of its use in medicinal, cosmetic and therapeutic, properties including anticancer effects. In this context, the present study will shed light upon the protective influence of Aloe vera against the deleterious effects induced by simultaneous exposure of radiation and cadmium in the brain of Swiss albino mice. For the purpose, six to eight weeks old male Swiss albino mice were selected and divided into seven groups:- Group I (Sham-irradiated ), Group II (treated with cadmium chloride 20ppm), Group III (Irradiated with 3.0 Gy and 6.0 Gy gamma rays), Group IV (Both irradiated and treated with cadmium chloride solution), Group V (Cadmium chloride and Aloe vera treated), Group VI (radiation and Aloe vera treated), Group VII (radiation, and cadmium chloride and Aloe vera treated). The animals were sacrificed at each post-treatment intervals of 1,2,4,7,14 and 28 days. The brain was taken out and quantitatively analyzed for different biochemical parameters such as total proteins, glycogen, cholesterol, acid phosphatase activity, alkaline phosphatase activity, DNA and RNA. The value of cholesterol, glycogen, RNA, acid phosphatase activity, and alkaline phosphatase activity increased up to day-14 in non drug-treated groups and day-7 in Aloe vera treated groups and thereafter decreased up to the last autopsy interval studied. The value of tota I proteins and DNA decreased up to day-14 in non drug-treated groups and day-7 in the drug treated groups then increased in all groups. In only cadmium chloride (without and with drug) treated animals (Groups II and V) the value of cholesterol decreased during early intervals (days-14 and 7 respectively) and increased thereafter. Severe changes were observed after combined exposure to radiation and cadmium chloride showing synergistic effect. Aloe vera reduced the severity of damage and made the recovery process earlier and faster. At all the corresponding intervals the drug treated animals showed less severe biochemical changes and an earlier and faster recovery, which may be due to the protection provided by Aloe vera.
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REFERENCES

References: 

1. Fang Y, Yang S, Wu: Free radicals, antioxidant, and nutrition. Nutrition 2002; 18: 879-887.
2. Kamat JP, Boloor KK, Devasagayam TPA, Venkatachalam S: Antioxidant properties of Asparagus racemosus against damage induced by g-radiation in rat liver mitrochondria. J. Ethnopharmacol 2000; 71: 425-435.
3. Wang Y, Liu L, Pazhanisamy KS, Li H, Meng A, Zhou D: Total body irradiation causes residual bone marrow injury by induction of persistent oxidative stress in murine hematopoietic stem cells. Free Radic. Bio. Med 2010; 48: 348-356.
4. Degrove N: Carcinogenesis teratogenic and mutagenic effects of cadmium. Mutation Res
1981; 86: 115- 135.
5. Waalkes MP, Watkins JB, Klaassen CD: Cadmium exposure from smoking cigarettes variations with time and country where purchased. Toxicology and Applied Pharmacology 1983; 68: 3 : 392-398.
6. Adams RG, Crab tree N: Anosima in alkaline battery workers. Br J Ind Med1961;
18:216-221.
7. Pihl RO, Parkes M: Hair element content in learning disabled children. Science 1977; 198:
202-206.
Available Online at www.ijprbs.com
Research
Articl
e CODEN: IJPRNK
Purohit RK, IJPRBS, 2013; Volume 2(4): 117-130
ISSN: 2277-8713
IJPRBS
8. Ozonas RB, Bstombo MCO, Santos-Ruiz A: Trace element metabolism in Animal-2.(W.G., Hoaekstra, J.W., Suttle,H. Ganther and W. Mertz.,(edu), University parkpress, Baltimore 1974; 476-478.
9. Rozear R, Degroof R, Somjen G: Effect of micro-iontophoretic administration of divalent metal ions on neurons of the central nervous system of cats. J Pharmacol Exp
Therp 1971; 176-118.
10. Cooper GP, Chandhasy H, Hastings H, Petering HG: Development toxicology of energy-related pollutants. DD Mahlum, MR Sibovi, PL Hockett and FD Andros (edu), U.S. Department of Energy, Oak ridge: 1978;627-637.
11. Jajetia GC: Radioprotective potential of plants and herbs against the effects of ionizing radiation. J Clin Biochem Nutr 2007;
40(2): 74-81.
12. Gehlot P and Goyal PK: Radioprotective effects of Aloe vera leaf extract on Swiss albino mice against whole-body gamma irradiation. Environmental Pathology: Toxicology and Oncology 2009; 28(1):1.
13. Ahaskar M and Sisodia R: Modulation of radiation induced biochemical changes in brain of swiss albino mice by Grewia astatica.
Asian J Exp Sci 2006; .20: 399-404.
14. Lowry OH, Rosenbrough MJ, Ferr AL and Randall RJ: Protein measurement with the folin-phenol reagent. J Bio Chem 1951; 193¬265.
15. Montgomery R: Determination of glycogen. Arch Biochem Biophys 1957; 67:
378.
16. Oser BL: Hawk's physiological chemistry, 14th Edn McGraw Hill, pp. 1154, New Delhi.
17. Fiske CH and Subbarow Y: The colorimetric estimation of phosphates. J Bio
Chem 1925; 66: 375.
18. Ceriotti G: Determination of nucleic acid in animal tissue. J Chem 1955; 214-59.
19. Ceriotti G.: The microchemical determination of deoxyribonucleic acid. J Bio
Chem 1952; 198-297.
20. Ahaskar M, Sharma KV, Singh S and Sisodia R: Post treatment effect of Grewia asiatica against radiation induced biochemical changes in brain of Swiss albino mice. Iran J. Radiat Res 2007; 5(3) 105-112.
21. Miquel J, Klatzo I, Menzel DB, Heymaker W: Glycogen changes in X-irradiated brain. Acta Neugopath 1963; 2: 482.
22. Monnier M and Krupp P: Action of gamma radiation on electrical brain activity in response of nervous system to ionizing radiation (T.J. Haley and R.S. Snider Eds.), Academic Press New York & London 1962;
607-620.
23. Nair V: Effects of exposure to low doses of x-radiation during pregnancy on the development of biochemical systems in the offspring in "Radiation Biology of the Fetal and Juvenile Mammal" (Eds Sikov MR and
Available Online at www.ijprbs.com
Research
Articl
e CODEN: IJPRNK
Purohit RK, IJPRBS, 2013; Volume 2(4): 117-130
ISSN: 2277-8713
IJPRBS
Mahlum DD) Atomic Energy Commission Office of Information Services 1969; 899-912.
24. Purohit RK, Joshi PK, Basu A, Agarwal M, Chakrawarti A and Bhartiya KM: Modulation influence of Emblica officinalis Iinn. On radiation and cadmium induced biochemical alterations in the brain of swiss albino mice. Pharmacologyonline 2010; 3: 304-316.
25. Wexlar BC, Pencharz R, Thomas SF: Adrenal ascorbic acid and histological changes in male and female rats after half body X-rays irradiation. Amer J Physiol 1955; 183: 71-74.
26. Nagler K. March D, Pfriegar FW: Glia derived signals induced synapse formation in neurons of the rat central nervous system. J Physiology 2001; 533: 665-67.
27. Yadav R: Possible role of Calcium antagonists in modifying brain lesions induced by heavy metal and ionizing radiation in mice. PhD thesis, University of Rajasthan, Jaipur, 2003.
29. Yadav R, Jindal A and Goyal PK: Protective action of Diltiazem against cadmium induced biochemical changes in the brain of swiss albino mice. Annals of neurosciences 2005;12: 13-31.
30. Highman B, Hanks AR: Serum intestinal alkaline phosphatase in rats after 800R whole body regional X-irradiation. Soc Exp Biol Med
1970; 133: 1201.
32. Baijal K: Biochemical and histopathological effects of external
irradiation of small intestine of mammals. PhD thesis, University of Rajasthan, Jaipur,
1978.
33. Akoev IG, Pashovkina MS, Dolgacheva LP, et al: Enzymatic activity of some tissues and blood serum from animals and humans exposed to microwaves and hypothesis on the possible role of free radical process in nonlinear effects and modification of emotional behaviour of animals. Radiat Biol
Radioecol 2002; 42: 322-30.
34. Lynn KR, Skinner WJ: Radiolysis of an alkaline phosphatase. Radiai Res. 1979; 57:
358.
35. Gajawat S: Role of vitamins in altering the hepatic toxicity against combined administration of lead radiation in mice: a histopathological and biochemical approach. PhD. thesis, University of Rajasthan, Jaipur,
2000.
36. Beck F, Lloyd JB, Squier C: A Laboratory hand book, Amersterdam: North Holland Publishing Co. 1975.
37. Mathur A and Sharma J: Prevention of radiation-induced biochemical changes in mouce brain by Punica granatum. IJPRBS
2012; 4: 320-338.

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