DEVELOPMENT OF VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF BETAXOLOL HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM

In this present research work we have developed a validated UV spectrometry method for estimation of Betaxolol Hydrochloride in pure and pharmaceutical dosage form. The developed method is accurate, cost efficient, fast and reproducible for the estimation of Betaxolol Hydrochloride in pure and pharmaceutical dosage form. Based on measurement of absorption of UV light, the spectra of Betaxolol Hydrochloride in simulated tear fluid as a solvent showed maximum absorption wavelength (λ max) at 224 nm. The calibration curve was plotted over the concentration range from 2-200 μg/ml of Betaxolol Hydrochloride with correlation coefficient 0.996. Validation was performed as per ICH Q2 guidelines for linearity, accuracy, precision, and recovery. This method has good reproducibility with % RSD less than one. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.2740 and 0.8305 respectively by simple UV spectroscopy. Thus this proposed validated method can successfully apply for estimation of Betaxolol Hydrochloride in quality control, routine analytical work, and in pharmaceutical dosage forms.