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Koyunda Kas Gelişimi Üzerine Bir Çalışma

A Study on Ovine Muscle Development

Journal Name:

  • İstanbul Üniversitesi Veteriner Fakültesi Dergisi

Publication Year:

  • 2012

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The ontogeny of ovine muscle was studied. 23 single fetuses aged between 48 and 125 days of gestation (dg) were collected from abattoirs. The weight and crown-rump length of fetuses were measured and gestational age was estimated. Semitendinosus muscle (ST) or hind limbs (for smallest fetuses) were dissected and stained for alkali-stable ATPase and slow myosin heavy chain (MHC) antibody and also examined under the electron microscope (EM). Based on histochemical, immunohistochemical and electron microscopy study, qualitative findings were obtained. The results suggested that some secondary fibres early express slow MHC. These small, slow expressing secondary fibres adjacent to the large, slow expressing primary fibres began to occur at 52 dg and these increased in size at aro und day 69 and then migrated around 87 dg to act as a scaffold for the next generation of secondary (tertiary) fibres. We conclude that some of the large, central, slow expressing (also less intensely stained with alkali-ATPase) fibres at near term sheep fetus might be secondary fibres.
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Abstract (Original Language): 
Koyunda kas ontojenisi üzerine çalışıldı. Gebelik yaşları (gy) 48 ile 125 arasında değişen 23 adet tekiz fötus mezbahadan toplandı. Fötusların ağırlıkları ve baş-kuyruk mesafeleri ölçülerek gebelik yaşları tahmin edildi. Semitendinosus kası (ST) veya çok küçük olan fötuslardan tüm arka bacak disekte edilerek alkali- adenozin trifosfataz (ATPaz) ve yavaş-miyozin ağır zincir (MHC) antikoru ile boyandı. Ayrıca, kesitler elektron mikroskopta (EM) incelendi. Histokimyasal, immunohistokimyasal ve EM çalışmalarına dayanarak kalitatif bulgular elde edildi. Sonuçlar, bazı sekonder liflerin çok erken dönemde yavaş MHC ekspresyonu yaptıklarını gösterdi. Büyük, yavaş MHC eksprese eden primer liflerin çevresinde oluşan bu küçük, yavaş MHC ekspresyonu yapan sekonder lifler 52. günde oluşmaya başlar; yaklaşık 69. günde çapları iyice artar ve yaklaşık 87. günde göç ederek ikinci generasyon sekonder liflerin (tersiyer) oluşumu için çerçeve görevi görürler. Gelişmiş geç dönem koyun fötusunda görülen büyük, merkezi ve yavaş MHC eksprese eden (alkali ATPaz ile düşük yoğunlukta da boyanan) liflerin bir kısmının aslında sekonder lifler olabileceği sonucuna varıldı.
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  • 2
97-106
  • Spanish

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