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DEVELOPMENT OF AN RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION AND FORCE DEGRADATION OF CEFIXIME AND MOXIFLOXACIN IN BULK AND PHARMACEUTICAL DOSAGE FORM

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2012

Key Words:

Author NameUniversity of Author
Abstract (2. Language): 
A simple, efficient, and reproducible RP-HPLC method for the simultaneous determination of Cefixime and Moxifloxacin in bulk and in pharmaceutical formulations has been developed and validated. The separation was carried out on Phenomix C18 (250x4.6 mm i.d, 5 um) column using acetonitrile: 0.08M potassium dihydrogenortho phosphate (adjusted to pH 8 with NaOH) in the ratio of 40:60 v/v as eluent. The flow rate was 1 ml/min and effluent was detected at 290 nm. The retention time of Cefixime and Moxifloxacin were 2.157 and 3.570 min. respectively. The linear dynamic range was 20-80 ug/ml and 20-80 ug/ml for Cefixime and Moxifloxacin, respectively. Percentage recoveries for Cefixime and Moxifloxacin were 98.50 + 0.25% and 99.00+ 0.25%, respectively. All the analytical validation parameters were determined and found in the limit as per ICH guidelines, which indicates the validity of the method. The developed method is also found to be precise and robust for the simultaneous determination of Cefixime and Moxifloxacin in tablet dosage forms.
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REFERENCES

References: 

1. Maryadele
JO
: The Merck Index: Encyclopedia of Chemicals, drugs and biological, 14th Ed. New Jersey: Published by Merck Research Laboratories, Division of Merck and Co., Inc. Whitehouse station: 2006: 1924.
2. Budavari S: The Merck Index: Encyclopedia of Chemicals, drugs and biological, 13th Ed. New Jersey: Published by Merck Research Laboratories, Division of Merck and Co., Inc. Whitehouse station: 2001: 1097 and 1125.
Available Online At www.ijprbs.com
Research Article
Chirag Shah, IJPRBS, 2012; Volume 1(4): 128-147
3. http://meherpharmainnovation.blogspot .com/2010/07/moxifloxacincefixime.html
4. Dhoka MV: Simultaneous Estimation of Cefixime Trihydrate and Erdosteine in Pharmaceutical Dosage form by RP-HPLC method. International Journal of Chem Tech Research. 2010; 2: 79-87.
5. Gandhi Santosh V: A simple and sensitive RP-HPLC method for simultaneous estimation of Cefixime and Ofloxacin in combined tablet dosage form",International Journal of Pharmacy and Pharmaceutical Science. 2011; 3: 46-48.
6. Kumar Rajnish: Development of colorimetric method for the analysis of pharmaceutical formulation containing both Ofloxacin and Cefixime", International Journal of Pharmacy and Pharmaceutical
Sciences. 2011; 3: 178-179.
7. Patel Satish: Simultaneous Spectrophotometric Determination of Cefixime Trihydrate and Ofloxacin in Tablet
Dosage form. IRJPS. 2011: 105-108.
8. Pareek V: Role of Different Hydrotopic Agents in Spectrophotometric and Chromatographic estimation of Cefixime.
ISSN: 2277-8713
IJPRBS
International Journal of Pharma and Bio Science. 2010.
9. Kumar Hemanth AK: Simple and rapid liquid chromatography method for determination of moxifloxacin in saliva. Journal of Chromatography B. 2011: 3663¬3667.
10. Rathinavel G: A Validated RP - HPLC Method for Simultaneous Estimation of Cefixime and Cloxacillin in Tablets. Journal
of Chemistry. 2008; 5: 648-651.
11. Kumudhavalli MV: Development and Validation of Rp-Hplc Method For Simultaneous Determination Of Cefixime And Potassium Clavulanate In Tablet Dosage Form" International Journal of Pharma Recent Research. 2010; 2: 57-60.
12. Motwani Sanjay K: Validated spectrophotometric methods for the estimation of moxifloxacin in bulk and pharmaceutical formulations" Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2007: 250-256.
13. Gandhi LR: Absorption Ratio method for estimation of Moxifloxacin HCl & Ketorolac Tromethamine in their combined dosage form by UV-Visible Spectroscopy"
Available Online At www.ijprbs.com
Research Article
Chirag Shah, IJPRBS, 2012; Volume 1(4): 128-147
International Journal of Pharmaceutical Research and Development. 2011: 21-26.
14. Mishra M: Simple and Validated UV-Spectroscopic method for estimation of Moxifloxacin HCl in bulk and formulation. Journal of Global Pharma and Technology. 2010.
15. Sahu: Spectrophotometric Estimation of Moxifloxacin in
Bul
k and its Pharmaceutical Formulations. Pharmacology online. 2011:
491-502.
16. Nimmagadda Srinivas: Development and validation of a HPLC method for simultaneous quantitation of gatifloxacin, sparfloxacin and moxifloxacin using levofloxacin as internal standard in human
plasma. Wiley. 2008: 1288-1295.
17.
Kuma
r Hemanth AK: Simple and rapid liquid chromatography method for
ISSN: 2277-8713
IJPRBS
determination of moxifloxacin in plasma. Journal of Chromatography B, 2009: 1205¬1208.
18. Rama Subbaiah: Method Development and Validation for estimation of Moxifloxacin HCl in tablet dosage form by RP-HPLC method. Pharmaceutica Analytica
Acta. 2010.
19.
Q
2 (R1), Text on Validation of Analytical Procedures, International Conference on Harmonization, Geneva, November 2005,
pp. 8-17.
20. Robert A. Nash and Alfred HW: In
Pharmaceutical Validation, 3rd Edn, Informa
Publishers, 2003: 693-99.
21. Beckett AH and Stenlake JB: Practical pharmaceutical chemistry, 4th ed., Delhi: CBS publishers and distributors; 1997.
Available

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