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Yaş ve Sigara içiminin Eritrosit Katalaz Aktivitesi ve Bazı Hematolojik Parametreler Üzerine Etkisi

The Effect Of Age And Smoking On Erythrocyte Catalase Activity And Some Hematological Parameters

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Abstract (2. Language): 
Catalase (CAT , EC 1.11.1.6) is an antioxidant enzyme that catalyses the conversion of hydrogen peroxide (H2O2) to water and V2 O2. The catalase shows various catalitic activity in different tissues. The enzyme activity is higher in liver and kidney and the lower in connective tissues. Human erythrocytes are rich in catalase. In this study, we determined CAT activities in erythrocytes from 475 healthy subjects attended to different clinics for different complaints. We have also divided the subjects into smoker (n=88) and nonsmoker groups (n=386) in order to determine whether smoking may affect the enzyme activity or not in erythrocytes. Enzyme activity was determined according to the method of Aebi. Activity was expressed in rate constant per gram hemoglobin (k/grHb). Besides CAT activity; RBC, MCV, MCHC, MCH and HCT values were also obtained from Coulter autosampler. In order to determine CAT activity in different age groups, subjects were divided into to four groups as follows: Group 1: ages between 0-20 (n=28), Group 2: ages between 21-40 (n=192), Group 3: ages between 41¬60 (n=172), Group 4: ages between 61-80 (n=83) Erythrocyte CAT activities of these groups were found to be 2981 ± 962 k/gHb, 3039 ± 923 k/gHb, 3086±797 k/gHb and 3266 ± 13 k/gHb, respectively. Although differences of the mean activities between the groups were not statistically significant, CAT activities were increased by age. There is no effect of smoking on CAT activity in erytrocytes. The activities were 31 73 ± 91 0 k/gHb for smokers,and 3065 ± 979 k/gHb for non-smokers. As seen, smokers have higher CAT activity than nonsmokers, but it was not statistically significant.
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Abstract (Original Language): 
Katalaz (CAT, EC 1. 11.1.6), hidrojen peroksitin suya dönüşümünü katalizleyen antioksidan bir enzimdir. Dokulardaki katalaz aktivitesi büyük oranda değişiklik gösterir. Enzim aktivitesi karaciğer ve böbrek dokularında yüksek düzeyde iken, bağ dokusunda düşüktür. Normalde insan eritrositleri katalaz bakımından zengindir. Bu çalışmada, bazı şikayetlerle farklı kliniklere başvuran 475 sağlıklı bireyden alınan eritrositlerdeki katalaz aktiviteleri belirlendi. Ayrıca sigara içiminin eritrositlerdeki enzim aktivitesine etkisini araştırmak için sağlıklı bireyler sigara içenler (n=88) ve sigara içmeyenler (n=386) olmak üzere iki gruba ayrıldı. CAT enzi m aktivitesi Aebi metoduna göre belirlendi. CAT aktivitelerinin yanında;kan sayım cihazı ile RBC, MCV, MCHC ve HCT parametreleri ölçüldü. Farklı yaş gruplarındaki katalaz aktivitelerini belirlemek için, bireyler dört gruba ayrıldı: 1. grup: yaşı 0-20 arasında olanlar (n=28), 2. grup: yaşı 21-40 arasında olanlar (n=192), 3. grup: yaşı 41-60 arasında olanlar (n=172), 4. grup: yaşı 60-81 arasında olanlar (n=83) Bu grupların eritrosit CAT aktiviteleri sırasıyla, 2981 ± 962 k/grHb, 3039 ± 923 k/grHb ve 3086+797, 3266 ± 1 3 k/grHb olarak hesaplandı. Yaş arttıkca katalaz aktivitesinde bir dereceye kadar artış gözlenirken, gruplar arasında istatistiki olarak anlamlı bir fark gözlenmedi. Sigara içiminin eritrosit katalaz aktivitesine etkisi olmadığı görüldü. Sigara içenlerde katalaz aktivitesi 31 73 ± 91 0 k/gHb iken, sigara içmeyenlerde 3065 ± 979 k/gHb bulundu. Görüldüğü gibi katalaz aktivitesi sigara içenlerde içmeyenlere göre biraz yüksektir fakat bu istatistiki olarak anlamlı değildir.
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