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ANTIMICROBIAL RESISTANCE PROFILE AND CHARACTERISATION OF ENTEROCOCCUS SPECIES FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL

Journal Name:

  • International Journal of Medical Research & Health Sciences

Publication Year:

  • 2013
DOI: 
10.5958/j.2319-5886.2.3.112

Key Words:

Author Name
Abstract (2. Language): 
Background: Enterococcus is one of the leading causes of nosocomial infections, with E faecalis and E faecium accounting up to 90-95% of clinical isolates. During recent years, the occurrence of other Enterococcal species from clinical samples increased with the properties of resistance to many antibiotics. Thus appropriate identification of Enterococci at species level is crucial for the management and prevention of these bacteria in hospital settings. Hence, this study was undertaken to highlight the incidence of multi drug resistant enterococcal species from various samples from human infections, in a tertiary care hospital. Methods: This work was conducted in our institution from January 2009 to December 2011. About 112 enterococcal isolates from various clinical specimens were included in the study. The isolates were identified by standard microbiological methods. Antimicrobial susceptibility testing was carried out by using Kirby-Bauer disc diffusion method. The prevalence of High level Gentamicin resistance was identified. Vancomycin resistance was assessed by E-test. Result: The commonest species identified was E faecalis (87.5%), followed by E faecium (8.9%). 14% of isolates produced beta haemolysis and gelatinase. 15% and 24% were the haemolytic and gelatinase producing enterococci. High level resistance was shown towards tetracycline, Amikacin, Cholramphenicol. Vancomycin resistance was identified in single isolate. Conclusion: There is achange in isolation pattern of enterococcal species. Besides, there is an increased rate of infection with multidrug resistant enterococci species, which necessitates frequent antimicrobial surveillance.
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  • 3
328-333
  • English

REFERENCES

References: 

1. Murray BE. Diversity among multidrugresistant
enterococci. Emerging Infectious
Diseases 1997; 4: 37–47.
2. Donskey CJ, Chowdhry TK, Hecker MT,
Hoyen CK, Hanrahan JA, Hujer AM et al.
Effect of antibiotic therapy on the density of
vancomycin-resistant enterococci in the stool
of colonized patients. New England Journal
of Medicine 2000; 343:1926–32.
3. Patel R. Clinical impact of vancomycinresistant
enterococci. J Antimicrob
Chemother 2003; 51 (3): 13–21.
4. Desai PJ, Pandit D, Mathur M, Gogate A.
The prevalence, identification and the
distribution of various species of enterococci
which were isolated from clinical samples,
with special reference to the urinary tract
infections in catheterized patients. India J
Med Microbial 2001; 19: 132-37.
5. Carmeli Y, Eliopoulos G, Mozaffari, E., &
Samore M. Health and economic outcomes of
vancomycin-resistant enterococci. Arch
Intern Med 1997; 162, 2223-28.
6. Ross PW. Streptococci and Enterococci.
Mackie and Mccartney’s Practical Medical
Microbiology, 14th edition. Elsievier, 2006;
268-69.
7. Murray BE. The life and the times of the
Enterococci. Clin Microbiol Rev 1990;3:46-
65.
8. Facklam RR. Recognition of the Group D
Streptococcal species of human origin by
doing biochemical and physiological tests. J
Appl Microbiol 1972; 23(6):1131-39.
9. Chow JW, Thal LA, Perri MB, Vazquez JA,
Donabedian SM, Clewell DB. Plasmidassociated
hemolysin and aggregation
substance production contribute to virulence
in experimental enterococcal
endocarditis. Antimicrob Agents
chemother 1993;37: 2474–7.
10. The Clinical and Laboratory Standards
Institute. Performance standards for
antimicrobial susceptibility testing, Wayne,
PA.17 the informational supplement; 2007;
M100-S17.
11. Ruoff LK, Spargo DJ, Ferraro JM. The
species identification of the Enterococci
isolates from clinical specimens. J Clin
Microbiol 1990; 28: 435-37.
12. Vandamme P, Vercauteran E, Lammens C
,Pensart N, Ievan M, Pot B et al. Survey of
Enterococcal Susceptibility Pattern in
332
Saraswathy et al., Int J Med Res Health Sci. 2013;2(3):328-333
Belgium. J Clin Microbial 1996; 34: 2572-
76.
13. Sreeja S, Sreenivasa Babu PR, Prathab AG.
The prevalence and characterization of the
Enterococcus species from various clinical
samples in a tertiary care hospital. J Clin
Diagn Res. 2012; 6(9): 1486–88.
14. Jain S, Kumar A, Kashyap B, Kaur RI. The
clinico-epidemiological profile and the highlevel
aminoglycoside resistance in
enterococcal septicemia at a tertiary care
hospital in east Delhi. Int J App Basic Med
Res 2011;1(2):80-83.
15. Vittal P P, Sambasiva R R and Subash C P.
Emergence of unusual species of enterococci
causing infections, South India. BMC
infectious diseases 2005,5:14
16. Facklam RR, Teixeira LM. Enterococcus. In:
Lollier L, Balows A, Sussman M, editors.
Topley & Wilson’s microbiology and
microbial infections. 9th ed. New York:
Oxford University Press; 1998. p. 669-82.
17. Bhat KG, Paul C, Ananthakrishna NC. Drug
resistant enterococci in a south Indian
hospital. Trop Doct 1998; 28:106-7.
18. Facklam RR., Sahm DF. & Teixeira LM.
Enterococcus. In Manual of Clinical
Microbiology, 7th edn 1999; pp. 297–305.
ASM Press, Washington, DC, USA.
19. Jones RN, Sader HS, Erwin ME, Anderson
SC. Emerging multiply resistant enterococci
among clinical isolates. I. Prevalence data
from 97 medical centre surveillance study in
the United States. Enterococcus Study Group.
Diagn Microbiol Infect Dis 1995; 21:85-93.
20. Kapoor L, Randhawa VS, Deb M.
Antimicrobial resistance of enterococcal
blood isolates at a pediatric care hospital in
India. Jpn J Infect Dis 2005; 58 : 101-3.

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