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Hemodiyaliz, Periton Diyalizi ve Prediyaliz Hastalarda Gizli Hepatit B Enfeksiyonunun Polimeraz Zincir Reaksiyonu Yöntemi ile Araştırılması

The Investigation of Occult Hepatitis B Infection in Hemodialysis, Peritoneal Dialysis, and Predialysis Patients by the Polymerase Chain Reaction Method

Journal Name:

  • Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi

Publication Year:

  • 2009

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The presence of hepatitis B virus DNA in case of negative hepatitis B surface antigen in serum is known as occult hepatitis B. Although the exact prevalance is not known, there are many reports indicating that occult hepatitis B is more frequently encountered in patients undergoing hemodialysis treatment. The aim of this study was to investigate the prevalence of occult hepatitis B in patients on hemodialysis or peritoneal dialysis, or with predialytic chronic renal failure. A total of 174 HBsAg-negative sera were included in the study. HBV-DNA was detected by polymerase chain reaction (PCR) in 2.6% of hemodialysis patients and 1.8% of peritoneal dialysis patients. In contrast, there was no occult hepatitis B virus infection in non-dialyzed patients with chronic renal failure. According to the liver enzymes and serum C-reactive protein levels, there was no statistically difference between the occult hepatitis B positive patients and the negative patients. In conclusion, although the rate was low, occult hepatitis B infections was detected among dialysis patients in our study. Therefore, despite serological analysis, both hemodialysis and peritoneal dialysis patients should be screened for occult HBV infections with a more sensitive test such as PCR, in order to prevent the dialysis-mediated transmision risk of HBV infection.
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Abstract (Original Language): 
Serumda hepatit B yüzey antijeninin (HBsAg) negatif olduğu durumlarda, hepatit B virüsü DNA (HBV-DNA)’sının varlığı gizli hepatit B olarak bilinmektedir. Gizli hepatit B’nin prevalansı tam olarak bilinmemesine rağmen, hemodiyaliz tedavisi uygulanan hastalarda daha sık olduğunu bildiren çok sayıda araştırma vardır. Bu çalışmada, hemodiyaliz ve periton diyalizi hastaları ile kronik böbrek yetersizliği olup henüz diyaliz tedavisi başlanmamış hastalarda (prediyaliz) gizli hepatit B prevalansı araştırıldı. Çalışmaya HBsAg-negatif olan toplam 174 hasta alındı. Polimeraz zincir reaksiyonu (PZR) yöntemi ile hemodiyaliz hastalarının %2.6’sında, periton diyalizi hastalarının %1.8’inde HBV-DNA varlığı saptandı. Buna karşılık, prediyalitik hastalarda gizli hepatit B enfeksiyonu saptanmadı. Gizli HBV enfeksiyonu saptanan hastalarla diğer hastalar arasında karaciğer enzimlerinin yüksekliği ve serum C-reaktif protein seviyesi yönünden anlamlı düzeyde farklılık saptanmadı. Sonuç olarak, çalışmamızda hemodiyaliz uygulanan hastalarda gizli HBV enfeksiyonunun düşük sıklıkla olsa da görülebileceği saptanmıştır. Hem hemodiyaliz hem de periton diyalizi uygulanan hastalara cihaz kaynaklı HBV bulaşımını önlemek için sadece serolojik testlerle virüs varlığının araştırılmasının yeterli olamayacağı, PZR gibi duyarlılığı daha yüksek testlerle bu hastaların taranmasının uygun olabileceği sonucuna varılmıştır.
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  • Turkish

REFERENCES

References: 

1. Zhang YY, Guo LS, Li L, Zhang YD, Hao LJ, Hansson BG,
Nordenfelt E: Hepatitis B virus DNA detected by PCR in sera and
liver tissues of Chinese patients with chronic liver diseases Chin
Med J 1993;106:7-12
2. Boyacıoğlu S: Hemodiyaliz ve böbrek naklinde hepatitis B virus
enfeksiyonu. Kronik B ve delta hepatiti tanı ve tedavisi ‘ulusal
uzlaşma toplantısı’ III.Ulusal Hepatoloji Kongresi Kitabı 1999:50-
58
3. Erek E, Serdengeçti K, Süleymanlar G: Registry of the Nephrology,
Dialysis and Transplantation in Turkey: Registry 2004. İstanbul,
Art Ofset, 2005:19
4. Doğukan A, Taşkapan H, Güven M, Tokgöz B, Oymak O, Utaş
C: Prediyaliz, hemodiyaliz ve sürekli ayaktan periton diyalizi
hastalarında çift doz hepatit B aşısına yanıt. Türk Nefroloji Diyaliz
Hipertansiyon ve Transplantasyon Dergisi 1999;4:192-194
5. Hu KQ: Occult hepatitis B virus infection and its clinical
implications. J Viral Hepatol 2002 ;9:243–257
6. Ergünay K: Gizli (Okült ) Hepatit B enfeksiyonu. Mikrobiyol Bült
2005; 39: 241-249
7. Weinberger KM, Bauer T, Böhm S, Jilg W: High genetic variability
of the group-specific a-determinant of hepatitis B virus surface
antigen (HBsAg) and the corresponding fragment of the viral
polymerase in chronic virus carriers lacking detectable HBsAg in
serum. J Gen Virol 2000; 81: 1165-1174
8. Torbenson M, Thomas DL: Occult hepatitis B. Lancet Infect Dis
2002;2:479-486
9. Tassopoulos NC, Kuhns MC, Koutelov MG, McNamara Al,
Todoulos A: Quantitative detection of hepatitis B virus DNA in
sera from patients with acute hepatitis B. Dig Dis Sci 1993; 38:
2156-2162
10. Ağaçayak A, Yılmaz S, Bulut Y, Özdarendeli A, Seyrek A:
Investigation of HBV DNA obtained from sera and semen in
hepatitis B carriers. Viral Hepatit Dergisi 2004; 9: 130-134
11. Mathurin P, Mouquet C, Poynard T, Sylla C, Benalia H, Fretz C,
et al: Impact of B and C virus on kidney transplantation outcome.
Hepatology 1999;29:257-263
12. Cabrerrizo M, Bartolome J, De Sequera P, Caramelo C, Carreno
V. Hepatitis B virus DNA in serum and blood cells of hepatitis B:
surface antigen-negative hemodialysis and staff. J Am Soc Nephrol
1997;8:1443-1447
13. Beşışık F, Karaca C, Akyüz F, Horosanli S, Onel D, Badur S, et
al: Occult HBV infection and YMDD variants in hemodialysis
patients with chronic HCV infection. J Hepatol 2003;38:506-510
14. Dueymes JM, Bodenes-Dueymes M, Mahe JL, Herman B:
Detection of hepatitis B viral DNA by polimerase chain reaction in
dialysis patients. Kidney Int 1993;41:161-166
15. Altındiş M, Uslan İ, Çetinkaya Z, Yüksel S, Çiftçi IH, Demirtürk
N, et al: Hemodiyaliz hastalarının gizli hepatit B varlığı yönünden
araştırılması. Mikrobiyol Bült 2007;41:227-233
DOĞUKAN: Hemodiyaliz, Periton Diyalizi ve Prediyaliz Hastalarda
Gizli Hepatit B Enfeksiyonunun Polimeraz Zincir Reaksiyonu
Yöntemi ile Araştırılması
Cilt/Vol. 18, No, 2, 2009 Sayfa/Page 55-61
60
Türk Nefroloji Diyaliz ve Transplantasyon Dergisi
Turkish Nephrology, Dialysis and Transplantation Journal
61
16. Fabrizi F, Messa PG, Lunghi G, Aucella F, Bisegna S, Mangano
S, et al. Occult hepatitis B virus infection in dialysis patients: A
multicentre survey. Aliment Pharmacol Ther 2005;21:1341-1347
17. Yakaryilmaz F, Gurbuz OA, Guliter S, Mert A, Songur Y, Karakan
T, Keles H: Prevalence of occult hepatitis B and hepatitis C virus
infections in Turkish hemodialysis patients. Ren Fail 2006;28:729-
735
18. Minuk GY, Sun DF, Greenberg R, Zhang M, Hawkins K, Uhanova
J, et al: Occult hepatitis B virus infection in a North American adult
hemodialysis patient population. Hepatology 2004;40:1072-1077
19. Cabrerrizo M, Bartolome J, Caramelo C, Barril G, Carreno V:
Molecular analysis of hepatitis B virus DNA in serum and peripheral
blood mononuclear cells from hepatitis B surface antigen-negative
cases. Hepatology 2000;32:116-123
20. Grob P, Jilg W, Bornhak H, Gerken G, Gerlich W, Günther S, et al:
Serological pattern “anti- HBc alone”: report on a workshop. J Med
Virol 2000; 62: 450-455
21. Sheen IS, Liaw YF, Chu CM, Pao CC: Role of hepatitis C virus
infection in spontaneous hepatitis B surface clearance during
chronic hepatitis B virus infection. J Infect Dis 1992;165:831-834
22. Cacciola I, Pollicino T, Squadrito G, Cerenzia G, Orlando ME,
Raimondo G: Occult hepatitis B virus infection in patients with
chronic hepatitis C liver disease. N Engl J Med 1999;341:22-26
23. Khattab E, Chemin I, Vuillermoz I, Vieux C, Mrani C, Guillaud O,
et al: Analysis of HCV co-infection with occult hepatitis B virus in
patients undergoing IFN therapy. J Clin Virol 2005;33:150-157
24. Shih CM, Lo SJ, Miyamura T, Chen SY, Lee YH: Supression of
hepatitis B virus expression and replication by hepatitis C virus
core protein in HuH-7 cells. J Virol 1993;67:5823-5832
25. Kao JH, Chen PJ, Lai MY, Chen DS: Occult hepatitis B virus
infection and clinical outcomes of patients with chronic hepatitis
C. J Clin Microbiol 2002;1:4068-4071
26. Silva C, Goncales NS, Pereira JS, Escanhoela CA, Pavan MH,
Goncales FL: The influence of occult infection with hepatitis B
virus on liver histology and response to interferon treatment in
chronic hepatitis C patients. Braz J Infect Dis 2004; 8: 431-439
27. Goral V, Ozkul H, Tekes S, Sit D, Kadiroglu AK: Prevalence of
occult HBV infection in haemodialysis patients with chronic HCV.
World J Gastroenterol 2006; 12: 3420-3424
28. Carpenter PA, Huang ML, McDonald GB: Activation of occult
hepatitis B from seronegative patient after hematopoietic cell
transplant: a cautionary tale. Blood 2002;99:4245-4246
29. Haushofer AC, Hauer R, Brunner H, Köller U, Trubert-Exinger D,
Halbmayer WM, et al: No evidence of hepatitis B virus activity
in patients with anti-HBc antibody positivity with or without antihepatitis
C virus antibody positivity. J Clin Virol 2004;29:221-223
30. Abdelmalek MF, Pahsa TM, Zein NN, Persing DH, Wiesner
RH, Douglas DD: Subclinical reactivation of hepatitis B virus
in liver transplant recipients with past exposure. Liver Transpl
2003;9:1253-1257
31. Tanaka Y, Esumi M, Shikata T: Persistence of hepatitis B virus DNA
after serological clearance of hepatitis B virus. Liver 1990;10:6-
10
32. Fukuda R, Ishimura N, Niigaki M, Hamamoto S, Satoh S, Tanaka S,
et al: Serologically silent heapatitis B virus coinfection in patients
with hepatitis C virus-associated chronic liver diseases:clinical and
virological significance. J Med Virol 1999;58:201-207
33. Fabrizi F, Mangano S, Alongi G, Bisegna S, Finazzi S, Lunghi G,
Ponticelli C: Influence of hepatitis B virus viremia upon serum
aminotransferase activity in dialysis population. Int J Artif Organs
2003;26:1048-1055
34. Chazouilleres O, Mamish D, Kim M, Carey K, Ferrell L, Roberts
JP, et al: “Occult” hepatitis B virus as source of infection in liver
transplant recipients. Lancet 1994;343:142-146.
35. Marcellin P, Giostra E, Martinot-Peignoux M, Loriot MA, Jaegle
ML, Wolf P, et al: Redevelopment of hepatitis B surface antigen
after renal transplantation. Gastroenterology 1991;100:1432-1434
36. Blackberg J, Kidd-Ljunggren K: Occult hepatitis B virus after acute
self-limited infection persisting for 30 years without sequence
variation. J Hepatol 2000;33:992-997

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