SCREENING OF ANTIOXIDANT AND ANTIMICROBIAL POTENTIAL OF HAWTHORN (CREATAGUS MONOGYNA) FRUIT AND LEAVES EXTRACTS
Journal Name:
- Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi
Keywords (Original Language):
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Abstract (2. Language):
In the present study, water and methanol extracts of hawthorn (Creatagus monogyna) were studied for antioxidant and antimicrobial properties. The antioxidant properties of fruits and leaves were evaluated by determining 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The water and methanol extracts of both fruits and leaves have antioxidant potential, ranging from 3.7 % to 44 %. Antimicrobial activities of above extracts were also tested against clinical isolates of human pathogenic strains belonging to 4 bacteria, Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and 3 yeast species, Candida albicans, Candida glabrata and Candida parapsilosis, by disk-diffusion method. The minimal inhibition concentration (MIC) value of each active extracts was also determined. The most effective antibacterial activity was expressed by methanol extract of hawthorn leaves against Gram-positive bacteria S. aureus with 12mm inhibition zone and 0.625 mg/ml MIC value. None of the extracts had antifungal activity against tested human pathogenic clinical isolates.
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Abstract (Original Language):
Bu çalışmada, alıcın (Creatagus monogyna) metanol ve su ektraktlarının antibakteriyal ve antioksidant aktiviteleri belirlenmiştir. Meyve ve yapraklardaki antioksidant aktivite 1,1-diphenyl-2-picrylhydrazyl (DPPH) radikal süpürme yöntemiyle belirlenmiş %3.7 ile %44 arasında değişen oranlarda aktivite tespit edilmiştir. Antimikrobiyal aktivite ise, hastalardan izole edilmiş olan 4 tür (Enterobacter aerogenes, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus) bakteri ve yine 3 tür (Candida albicans, Candida glabrata and Candida parapsilosis) maya üzerinde disk diffüzyon yöntemi kullanılarak belirlenmiştir. Disk diffüzyon yöntemiyle aktivite tespit edilen bakteriler için minimal inhibisyon konsantrasyonları (MİK) da belirlenmiştir. En yüksek aktivite, alıç yaprağının metanol ekstraktından, S. aureus türüne karşı 12mm inhibisyon zonu ve 0.625 mg/ml MİK değeriyle elde edilmiştir. Çalışmamızda antifungal aktivite tespit edilememiştir.
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