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Hematopoietik kök hücre transplantasyonunda CD34 (+) hücre seleksiyonu ve ayıklama

CD34 (+) cell selection and purging in hematopoietic cell transplantation

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Abstract (2. Language): 
Peripheral blood stem cells have replaced bone marrow as the primary source for autologous stem cell transplantation. Although tumor burden appears to be lower in peripheral blood stem cell fractions than in the bone marrow, modern detection methods can detect the tumor cell present in peripheral blood stem cell fractions. Tumor cells in the graft may contribute to relapses in posttransplant period. Purging of peripheral blood stem cell fractions can be done either by negative or positive selection. CD34 positive selection has been successfully used to deplete tumor cells from grafts for autologous transplantation, to deplete T cells for allogeneic transplantation, to enrich progenitor cells for gene therapy and ex-vivo expansion strategies of progenitor cells for 15 years.
Abstract (Original Language): 
Periferik kan kök hücreler otolog kök hücre naklinde kök hücre kaynağı olarak kemik iliğinin yerini almaktadır. Tümör kitlesi her ne kadar periferik kan kök hücre fraksiyonlarında kemik iliğinden daha az olsa da, modern araştırma yöntemleri tümör hücrelerini periferik kan kök hücre fraksiyonlarında ortaya koyabilir. Graft içindeki tümör hücreleri posttransplant dö- nemdeki nükslere katkıda bulunabilir. Periferik kan kök hücre fraksiyonlarını tümörden ayıklama işlemi negatif ya da pozitif seleksiyonla yapılabilir. CD34 pozitif seleksiyon, on beş yıldan beri otolog transplantasyonda graftın tü- mör hücrelerinden temizlenmesinde, allojeneik transplantasyonda T hücre deplesyonunda, gen tedavisinde progenitör hücrelerin saflaştırılmasında ve progenitör hücrelerin ex-vivo ekspansiyonunda başarıyla kullanılmaktadır.
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