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EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF FRUIT CONSTITUENTS OF AMOMUM SUBULATUM ROXB

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Abstract (2. Language): 
Reactive oxygen Species (ROS) are highly toxic to cells. Cellular antioxidant enzymes and the free radical scavengers normally protect the cell from toxic effects of the ROS. The dietary ingredients of Indian food play an important role in preventing the generation of ROS. Amomum subulatum Roxb. One of the important spices in Indian food was evaluated for its antioxidant activity. Amomum fruits were extracted with acetone and methanol. Both the extracts were subjected to Ferric reducing power (FRAP) test and DPPH radical scavenging activity using Ascorbic acid as reference standard. Result in FRAP test shows significant efficacy as compared to ascorbic acid. The activity was found to be concentration dependant. The DPPH radical scavenging activity has shown that it was concentration dependent as with increasing concentration it was increasing. And IC50 value of both the extract was at 20 u.g /ml. Thus it has been concluded that spices like Amomum subulatum Roxb. Used in Indian food may be helpful in preventing oxidative stress.
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