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Hidrojen peroksidin dana koroner arter düz kasında gevşetici etki mekanizmaları

Mechanisms of relaxing effect of hydrogen peroxide on bovine coronary artery

Journal Name:

  • Selçuk Tıp Dergisi

Publication Year:

  • 2003

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
İn this in vitro study, the effects ofH202 on bovine coronary artery and roles of K+ channels and cyclooxygenase, nitric oxide synthase and Na+/K+-ATPase enzymes forthese effects were investigated. Strips from arteries were mounted in 25 ml organ baths containing Krebs-Henseleit Solution at 37 "C aerated with %95 02 and %5 Cö2. Effects of H202 on basal tone of tissues with and vvithout endothelium were investigated. İn the second part of study, tissues were contracted with U46619 (3x10-7 M) and H202 (1ö-7-10-2M) was added to the organ baths, cumulatively. This procedure was repeated on tissues incubated with apamin (10-6M), charybdotoxİn (1CT7M), tetraethylammonium (TEA, lO^M), glibenclamide (10-6M), indomethacin (10-5M), NG-nitro-L-arginine methyl ester (L-NAME, 1(yAM) and ouabaine (105 M). Each group of tissue was incubated with only one of these agents. H202 did not affect basal tone of tissues with and vvithout endothelium but relaxed tissues contracted with U46619 in con-centration dependent manner. İn tissues with endothelium and in tissues incubated with apamine, charybdotoxine and tetraethylammonium, Emax values and plC50 values of H202 vvere not different significantiy from values obtained from tissues vvithout endothelium (control tissues). Hovvever values obtained from tissues incubated with glibenclamide, indomethacin, L-NAME and ouabaine vvere different significantiy from values obtained from tissues vvithout endothelİum.These results indicate that ATP-sensitive potassium channels and cyclooxygenase, nitric oxide synthase and Na+/K+-ATPase enzymes play role in the relaxant effects of H2Ö2 on bovine coronary artery smooth muscle.
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Abstract (Original Language): 
Bu in vitro çalışmada, reaktif oksijen türlerinden H202'nin dana koroner arterindeki etkileri ve bu etkilerde K+ kanal¬ları ile siklooksijenaz, nitrik oksid sentaz ve Na+/K+-ATPaz enzimlerinin rolleri araştırılmıştır. Arterlerden elde edilen şeritler %95 02 -%5 C02 karışımı ile gazlandırılan, 37 "C'de Krebs-Henseleit solüsyonu içeren, 25 ml hac¬mindeki organ banyosu içine alındı. Endotelli ve endotelsiz dokularda H202'nin bazal tonus üzerine etkisi araştırıldı. Çalışmanın diğer bir bölümünde dokular U46619 (3x10~7M) ile kasıldı ve banyolara kümülatif tarzda H202 (10-7 - 10-2M) İlave edildi. Bu prosedür H202 ilavesinden önce apamin (1Cr6M), karibdotoksin (10-7M), TEA (10-4M), glibenklamid (1(r6M), indometazin (10-5M), L-NAME (tCr4M) ve uvabain (ia5M) ile inkübe edilen doku¬larda tekrarlandı. Her bir gruba bu ajanlardan yalnız biri uygulandı. H202 endotelli ve endotelsiz dokuların bazal tonusunu değiştirmedi. U46619 ile kasılan dokularda ise doza bağımlı tarzda gevşeme cevapları oluşturdu. Endotel tabakası sağlam olan ve apamin, karibdotoksin ve TEA ile inkübe edilen dokularda H202 için hesaplanan Emax ve plC50 değerleri ile endotelsiz (kontrol) dokulardan alınan değerler arasında anlamlı bir fark bulunmadı. Glibenklamid, indometazin, L-NAME ve uvabain İle İnkübe edilen dokulardaki değerler ise endotelsiz dokulardan alınan değerlere göre anlamlı olarak farklı idi. Bu sonuçlar H202'nin dana koroner arter düz kasında oluşturduğu gevşeme cevaplarında KATP tipi K+ kanalları ile siklooksijenaz, nitrik oksid sentaz ve Na+/K+-ATPaz enzimlerinin aktivasyonunun rol oynadığını göstermektedir.
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  • 2
71-78
  • Turkish

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