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DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF LEVOFLOXACIN HEMIHYDRATE AND AMBROXOL HYDROCHLORIDE IN THEIR COMBINED DOSAGE FORM

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2013

Key Words:

Author Name
Abstract (2. Language): 
A simple, specific, accurate, precise and economical spectrophotometric method has been developed for the estimation of Levofloxacin (LVF) and Ambroxol (AMB) in pharmaceutical dosage forms. Simultaneous equation method was used. The absorption maxima of the drugs were found to be 288 nm and 244 nm for Levofloxacin and Ambroxol respectively. Levofloxacin and Ambroxol obeyed Beer's law in the concentration range of 2- 16u.g/ml and 1-50u.g/ml respectively. Different analytical parameters such as linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ) were determined as per ICH guidelines. The % assay for commercial formulation was found to be in the range of 99-101%.The recovery values between prescribed limit of 98-102% shows that the method is free from interference of excipients present in formulation. Hence, the developed method can be used quality control analysis for routine.
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Shivani Chanda, IJPRBS, 2013; Volume 2(4): 311-322
ISSN: 2277-8713
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